Porous Poly(vinyl alcohol)-Alginate Gel Hybrid Construct for Neocartilage Formation Using Human Nasoseptal Cells
Porous Poly(vinyl alcohol)-Alginate Gel Hybrid Construct for Neocartilage Formation Using Human Nasoseptal Cells
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DOI：
10.1016/j.jss.2010.03.070
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发表时间：
2010-10-01
影响因子：
2.2
通讯作者：
Muratoglu, Orhun K.
中科院分区：
文献类型：
--
作者：
Bichara, David A.;Zhao, Xing;Muratoglu, Orhun K.
背景。颅面软骨修复的选择有限。自体组织或多孔聚乙烯目前用于鼻和耳廓重建。这两种选择都有缺点，包括供体部位发病率和植入物挤出。聚乙烯醇 (PVA) 是一种不可降解的柔性生物相容性聚合物，可以通过设计来模仿软骨的特性。本研究的目标是使用 PVA-藻酸盐水凝胶和人鼻中隔软骨细胞的组合来设计生物合成混合结构。材料和方法。从人鼻中隔软骨中分离的软骨细胞被扩增并与2%海藻酸钠水凝胶混合。将软骨细胞-藻酸盐混合物注入不可降解的多孔 PVA 水凝胶中，形成生物合成结构。将这些构建体中的一组植入裸鼠的皮下环境中，而另一组在转瓶生物反应器系统中培养10天然后植入。体内6周后，检查组织学、生化和生物力学特性。结果。组织学分析表明两组构建体中均存在硫酸化糖胺聚糖和 II 型胶原沉积。在体内植入之前在生物反应器系统中培养的构建体表现出更高水平的DNA、糖胺聚糖和羟脯氨酸。还观察到暴露于生物反应器的工程构建体的抗压强度增加了 22%。结论。一种新型多孔 PVA-海藻酸盐凝胶混合物被用来成功地在体内改造人类软骨。生物反应器培养 10 天后，DNA、糖胺聚糖、羟脯氨酸和构建体的压缩模量水平均有所提高。 (C) 2010 Elsevier Inc. 保留所有权利。
Background. Limited options exist for the restoration of craniofacial cartilage. Autologous tissue or porous polyethylene is currently used for nasal and auricular reconstruction. Both options are associated with drawbacks, including donor site morbidity and implant extrusion. Poly(vinyl alcohol) (PVA) is a non-degradable flexible biocompatible polymer than can be engineered to mimic the properties of cartilage. The goal of this study was to engineer a biosynthetic hybrid construct using a combination of PVA-alginate hydrogels and human nasal septum chondrocytes.Materials and Methods. Chondrocytes isolated from human nasal septum cartilage were expanded and mixed with 2% sodium alginate hydrogel. The chondrocyte-alginate mix was injected into a non-degradable porous PVA hydrogel, creating biosynthetic constructs. A group of these constructs were implanted into the subcutaneous environment of nude mice, while the other group was cultured in a spinner flask bioreactor system for 10 d and then implanted. After 6 wk in vivo, the histologic, biochemical, and biomechanical properties were examined.Results. Histological analysis demonstrated sulfated glycosaminoglycans and deposition of collagen type II in constructs from both groups. Constructs cultured in the bioreactor system prior in vivo implantation demonstrated higher levels of DNA, glycosaminoglycans, and hydroxyproline. An increase of 22% in the compressive strength of the engineered constructs exposed to the bioreactor was also observed.Conclusion. A novel porous PVA-alginate gel hybrid was used to successfully engineer human cartilage in vivo. A 10-d period of bioreactor culturing increased levels of DNA, glycosaminoglycans, hydroxyproline, and the compressive modulus of the constructs. (C) 2010 Elsevier Inc. All rights reserved.