Sex differences in the molecular signature of the developing mouse hippocampus.
Sex differences in the molecular signature of the developing mouse hippocampus.
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DOI：
10.1186/s12864-017-3608-7
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发表时间：
2017-03-16
期刊：
影响因子：
4.4
通讯作者：
Nowakowski RS
中科院分区：
文献类型：
--
作者：
Bundy JL;Vied C;Nowakowski RS
各种神经系统疾病，包括阿尔茨海默病、帕金森氏病、重度抑郁症、阅读障碍和自闭症，在女性和男性之间的患病率有所不同。为了更好地了解神经系统疾病性别偏向性的可能分子基础，我们使用了1、2和4个月大的雌性和雄性小鼠发育系列，分别使用rna测序和质谱法评估海马中的mRNA和蛋白质。转录组学分析确定了2699个基因在不同年龄的动物之间表达差异。这些差异表达基因的大部分在一个或多个年龄在两性中都发生了变化，但总共有198个转录本在一个或多个年龄在女性和男性之间存在差异表达。雌性和雄性之间差异表达的转录本数量在成年动物中比在年轻动物中要多。此外，我们鉴定了69个转录本，显示出通过出生后发育的复杂和性别特异性的时间调节模式，其中8个是热休克蛋白。我们还发现小鼠海马中mRNA和蛋白质水平之间存在适度的相关性（Rho = 0.53）。这项研究增加了大量的证据海马转录组调控在出生后的发展。此外，该分析揭示了发育中的小鼠海马转录组的性别差异，并进一步阐明了将雌性和雄性小鼠纳入涉及海马分子变化的纵向研究的必要性。本文的在线版本（doi:10.1186/s12864-017-3608-7）包含补充材料，可供授权用户使用。
A variety of neurological disorders, including Alzheimer’s disease, Parkinson’s disease, major depressive disorder, dyslexia and autism, are differentially prevalent between females and males. To better understand the possible molecular basis for the sex-biased nature of neurological disorders, we used a developmental series of female and male mice at 1, 2, and 4 months of age to assess both mRNA and protein in the hippocampus with RNA-sequencing and mass-spectrometry, respectively. The transcriptomic analysis identifies 2699 genes that are differentially expressed between animals of different ages. The bulk of these differentially expressed genes are changed in both sexes at one or more ages, but a total of 198 transcripts are differentially expressed between females and males at one or more ages. The number of transcripts that are differentially expressed between females and males is greater in adult animals than in younger animals. Additionally, we identify 69 transcripts that show complex and sex-specific patterns of temporal regulation through postnatal development, 8 of which are heat-shock proteins. We also find a modest correlation between levels of mRNA and protein in the mouse hippocampus (Rho = 0.53). This study adds to the substantial body of evidence for transcriptomic regulation in the hippocampus during postnatal development. Additionally, this analysis reveals sex differences in the transcriptome of the developing mouse hippocampus, and further clarifies the need to include both female and male mice in longitudinal studies involving molecular changes in the hippocampus. The online version of this article (doi:10.1186/s12864-017-3608-7) contains supplementary material, which is available to authorized users.