MICROTUBULE DYNAMICS IN-VITRO ARE REGULATED BY THE TUBULIN ISOTYPE COMPOSITION
MICROTUBULE DYNAMICS IN-VITRO ARE REGULATED BY THE TUBULIN ISOTYPE COMPOSITION
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DOI：
10.1073/pnas.91.24.11358
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发表时间：
1994-11-22
影响因子：
11.1
通讯作者：
WILSON, L
中科院分区：
文献类型：
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作者：
PANDA, D;MILLER, HP;WILSON, L
在体外稳定状态下，由等典型纯α β (II)、α β (III)或α β (IV)微管蛋白二聚体组装而成的单个牛脑微管的正端生长和缩短动力学，通过差示干涉对比视频显微镜进行了测定。由纯化的α β (III)同型组装的微管比由α β (II)或α β (IV)同型或未分离的磷酸纤维素纯化的微管更具活力。此外，在α β (II)和α β (III)同型混合物中增加α β (II)同型的比例可以抑制微管动力学，这表明微管动力学可以受到微管蛋白同型组成的影响。这些数据支持了一个假设，即细胞可能通过改变不同微管蛋白同种型的相对数量来部分地决定其微管的动态特性和功能。
The growing and shortening dynamics of individual bovine brain microtubules at their plus ends at steady state in vitro, assembled from isotypically pure alpha beta(II), alpha beta(III), or alpha beta(IV) tubulin dimers, were determined by differential interference contrast video microscopy. Microtubules assembled from the purified alpha beta(III) isotype were considerably more dynamic than microtubules made from the alpha beta(II) or alpha beta(IV) isotypes or from unfractionated phosphocellulose-purified tubulin. Furthermore, increasing the proportion of the alpha beta(II) isotype in a mixture of the alpha beta(II) and alpha beta(III) isotypes suppressed microtubule dynamics, demonstrating that microtubule dynamics can be influenced by the tubulin isotype composition. The data support the hypothesis that cells might determine the dynamic properties and functions of its microtubules in part by altering the relative amounts of the different tubulin isotypes.