AN ONTOGENIC STUDY OF KINDLING USING RAPIDLY RECURRING HIPPOCAMPAL SEIZURES
AN ONTOGENIC STUDY OF KINDLING USING RAPIDLY RECURRING HIPPOCAMPAL SEIZURES
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DOI：
10.1016/0165-3806(91)90116-z
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发表时间：
1991-07-16
期刊：
影响因子：
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通讯作者：
LOTHMAN, EW
中科院分区：
文献类型：
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作者：
MICHELSON, HB;LOTHMAN, EW
本研究探讨了未成熟大鼠点燃的获得和保留。出生后（PN）7-28天大的大鼠在腹侧海马中被电点燃。10秒，20 Hz的刺激列车提供每5分钟6小时的一天（短间隔快速复发海马癫痫发作，RRHS）或每30分钟9小时的每一个连续的两天（长间隔RRHS）。记录每次刺激后的放电持续时间（ADD）和行为发作评分（BSS）。所有年龄段的动物都以短和长间隔RRHS点燃，表现为ADD延长和BSS增加。短时间间隔RRHS，点燃的过程是不稳定的，与长时间间隔RRHS，点燃顺利进行了两个测试天。在PN 14-28大鼠中，在长间隔RRHS的第一天获得的点燃程度在第二个实验日开始时保留。相比之下，PN 7大鼠从第1天到第2天显示出ADD和BSS的一过性降低。后放电阈值随成熟而下降。在PN 14-28动物中，年轻大鼠在点燃开始时表现出更长的癫痫发作时间，并且更快地通过点燃。一旦建立，点燃运动癫痫发作也发生2秒，50赫兹的刺激列车。我们的结论是，快速点燃发生在所有年龄段，然而，PN 7大鼠保留点燃效应的能力比老年大鼠。
The present study investigated the acquisition and retention of kindling in immature rats. Postnatal (PN) 7-28-day-old rats were electrically kindled in the ventral hippocampus. Ten-second, 20-Hz stimulus trains were delivered every 5 min for 6 h on one day (short interval rapidly recurring hippocampal seizures, RRHS) or every 30 min for 9 h on each of two consecutive days (long interval RRHS). After discharge durations (ADD) and behavioral seizure scores (BSS) were recorded following each stimulation. Animals of all ages kindled with both short and long interval RRHS, as manifested by lengthening of ADD and increasing BSS. With short interval RRHS, the course of kindling was erratic; with long interval RRHS, kindling proceeded smoothly over both test days. In PN 14-28 rats, the degree of kindling obtained on the first day of long interval RRHS was retained at the start of the second experimental day. In contrast, PN 7 rats showed a transient decrease in ADD and BSS from day 1 to day 2. Afterdischarge thresholds declined with maturation. Among the PN 14-28 animals, younger rats exhibited longer seizures at the outset of kindling and proceeded through kindling faster. Once established, kindled motor seizures also occurred with 2-s, 50-Hz stimulus trains. We conclude that rapid kindling occurs at all ages; however, PN 7 rats are less capable of retaining the kindling effect than are older rats.