Light-quality regulation of freezing tolerance in Arabidopsis thaliana
Light-quality regulation of freezing tolerance in Arabidopsis thaliana
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DOI：
10.1038/ng.2007.3
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发表时间：
2007-11-01
期刊：
影响因子：
30.8
通讯作者：
Whitelam, Garry C.
中科院分区：
文献类型：
--
作者：
Franklin, Keara A.;Whitelam, Garry C.
为了获得抗冻性，高等植物需要一段时间的低温（通常< 4摄氏度），称为冷驯化。在将植物转移至低温后，转录激活因子的bar作用子（CBF）家族下bar RT/ DRE下bar标记（f）下bar标记（B）下bar标记（C）的表达增加导致含有C-重复/干旱响应（CRT/DRE）启动子元件的基因的上调和增强对零度以下温度的耐受性的代谢变化（1）。在这里，我们表明，一个低的红色远红外比（R/FR）光信号增加CBF基因表达的方式依赖于生物钟在拟南芥。CBF表达的这种光质量依赖性增加足以在高于冷驯化所需的温度下赋予抗冻性。此外，使用光质量信号刺激CBF表达揭示了CBF转录因子与下游冷调节（COR）基因的环境温度依赖性偶联，为该途径中的第二个温度调节步骤提供了证据。
To acquire freezing tolerance, higher plants require a period of low temperature ( usually < 4 degrees C) termed cold acclimation. Upon transfer of plants to low temperature, increased expression of the (C) under bar RT/ DRE (b) under bar inding (f) under bar actor (CBF) family of transcriptional activators leads to the upregulation of genes containing a C-repeat/drought-responsive (CRT/DRE) promoter element and metabolic changes that enhance tolerance to subzero temperatures(1). Here, we show that a low red to farred ratio (R/FR) light signal increases CBF gene expression in Arabidopsis thaliana in a manner dependent on the circadian clock. This light quality-dependent increase in CBF expression is sufficient to confer freezing tolerance at temperatures higher than those required for cold acclimation. Furthermore, the use of light-quality signals to stimulate CBF expression has revealed ambient temperature-dependent coupling of CBF transcription factors to downstream COLD REGULATED ( COR) genes, providing evidence for a second temperature-regulated step in this pathway.