miR2118-dependent U-rich phasiRNA production in rice anther wall development
miR2118-dependent U-rich phasiRNA production in rice anther wall development
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DOI：
10.1038/s41467-020-16637-3
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发表时间：
2020-06-19
影响因子：
16.6
通讯作者：
Komiya, Reina
中科院分区：
文献类型：
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作者：
Araki, Saori;Ngoc Tu Le;Komiya, Reina
生殖特异性小RNA是动物和植物生殖细胞发育的重要调控因子。microRNA 2118（miR 2118）在植物中是保守的，并诱导产生阶段性小干扰RNA（phasiRNA）。为了揭示miR 2118的生物学功能，我们在这里描述了miR 2118簇的大缺失的水稻突变体。我们的研究结果表明，miR 2118的损失导致严重的雄性和雌性不育水稻，与显着的形态和发育异常的体细胞花药壁细胞。小RNA分析显示，花药壁中的miR 2118依赖的21个核苷酸（nt）的phasiRNA富含U，与生殖细胞中的phasiRNA不同。此外，21-nt phasiRNA的miR 2118依赖的生物发生可能涉及Argonaute蛋白OsAGO 1b/OsAGO 1d，其在花药壁细胞层中丰富。我们的研究突出了体细胞花药壁和生殖细胞中phasiRNA的位点特异性差异，并证明了miR 2118/U-phasiRNA功能在花药壁发育和水稻生殖中的意义。MicroRNA 2118在植物中诱导阶段性小干扰RNA（phaisRNA）的产生。在这里，作者表明水稻miR 2118是雄性和雌性育性所必需的，并支持花药壁中丰富的非典型富含U的21 nt phasiRNA的产生。
Reproduction-specific small RNAs are vital regulators of germline development in animals and plants. MicroRNA2118 (miR2118) is conserved in plants and induces the production of phased small interfering RNAs (phasiRNAs). To reveal the biological functions of miR2118, we describe here rice mutants with large deletions of the miR2118 cluster. Our results demonstrate that the loss of miR2118 causes severe male and female sterility in rice, associated with marked morphological and developmental abnormalities in somatic anther wall cells. Small RNA profiling reveals that miR2118-dependent 21-nucleotide (nt) phasiRNAs in the anther wall are U-rich, distinct from the phasiRNAs in germ cells. Furthermore, the miR2118-dependent biogenesis of 21-nt phasiRNAs may involve the Argonaute proteins OsAGO1b/OsAGO1d, which are abundant in anther wall cell layers. Our study highlights the site-specific differences of phasiRNAs between somatic anther wall and germ cells, and demonstrates the significance of miR2118/U-phasiRNA functions in anther wall development and rice reproduction. MicroRNA2118 induces the production of phased small interfering RNAs (phaisRNAs) in plants. Here the authors show that rice miR2118 is required for both male and female fertility and supports the production of atypical U-rich 21nt phasiRNAs that are abundant in anther walls.