Emodin attenuates Alzheimer's disease by activating the protein kinase C signaling pathway
Emodin attenuates Alzheimer's disease by activating the protein kinase C signaling pathway
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DOI：
10.14715/cmb/2019.65.5.5
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发表时间：
2019-01-01
影响因子：
1.6
通讯作者：
Wang, Maode
中科院分区：
文献类型：
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作者：
Du, Changwang;Shi, Luoning;Wang, Maode
探讨大黄素对阿尔茨海默病（AD）模型小鼠学习记忆及蛋白激酶C（PKC）信号通路的影响。选择60只7-8月龄APP/PS1双转基因AD小鼠作为模型小鼠，36只健康雄性C57 BL/6小鼠作为对照组。采用Morris水迷宫法和被动回避实验评价小鼠的记忆能力。采用噻唑蓝（MTT）比色法和乳酸脱氢酶（LDH）细胞毒性试剂盒检测大黄素对β-淀粉样肽1-42（A β 1-42）处理的HT 22小鼠海马神经元细胞活力的影响。采用改良Takai法和Western blotting检测大黄素对PKC水平的影响。行为学测试结果显示模型组小鼠逃避潜伏期较对照组延长（P< 0.05），大黄素预处理组小鼠逃避潜伏期明显缩短。MTT和LDH实验结果显示大黄素对A β过表达的HT 22小鼠海马细胞有保护作用。Western blot分析显示，大黄素处理后，小鼠体内PKC磷酸化水平显著升高。大黄素可通过激活PKC通路减轻阿尔茨海默病模型小鼠的氧化应激和炎症反应，从而改善认知功能。
To investigate the effects of emodin on learning and memory and protein kinase C (PKC) signaling pathway in Alzheimer's disease (AD) model mice. 60 APP/PS1 double transgenic AD mice were selected as model mice at the age of 7-8 months, 36 healthy male C57BL/6 mice served as the control group. Morris water maze method and passive avoidance experiment were used to evaluate the memory ability of mice. The thiazole blue (MTT) method and the lactate dehydrogenase (LDH) cytotoxicity test kit were used to evaluate the effect of emodin on the cell viability of hippocampal neurons in HT22 mice treated with beta-amyloid peptide 1-42 (A beta 1-42). The effect of emodin on PKC levels was explored using the modified Takai method and Western blotting. Behavioral test results showed that the escape latency of the mice in the model group was longer than that in the control group (P< 0.05), and the escape latency was significantly shortened given a emodin prognosis. The MTT and LDH test results showed that emodin to A beta- overexpression induced the protective effect of hippocampus cells in HT22 mice. Western blot analysis showed that the phosphorylation level of PKC in mice increased significantly after emodin administration. Emodin can attenuate oxidative stress and inflammatory response in Alzheimer's model mice by activating PKC pathway, thereby improving cognitive function.