Characterisation of a chikungunya virus from a German patient returning from Mauritius and development of a serological test
Characterisation of a chikungunya virus from a German patient returning from Mauritius and development of a serological test
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DOI：
10.1007/s00430-008-0090-5
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发表时间：
2008-12-01
影响因子：
5.4
通讯作者：
Bodem, Jochen
中科院分区：
文献类型：
--
作者：
Kowalzik, Stefan;Xuan, Nghia Vu;Bodem, Jochen
我们从一名 2006 年春季访问毛里求斯三个月后返回德国的患者身上分离出了基孔肯雅 (Chik) 病毒。返回后，她出现短暂发烧，高达 40 摄氏度。随后出现肌肉痛和关节痛。血清中未检测到 Chik 病毒 IgG 抗体。在 Vero 细胞上分离出病毒 (Chik-Wu1)。我们通过 RT-PCR 从病毒 RNA 中分子克隆了 Chik-Wu1 的整个基因组。确定了完整的序列并指定了基因组的功能域。 Chik-Wu1 显然属于最近印度洋爆发的病毒组。为了开发可用于进一步表征 Chik-Wu1 的工具，我们通过细菌表达和纯化衣壳 (C) 和包膜 (E) 蛋白，并建立了免疫印迹测定法。来自 Chik 病毒感染患者的 30 份血清样本中，有 22 份先前在间接免疫荧光中得分呈阳性，在重组 C 和 E2 抗原的免疫印迹分析中也有反应。
We have isolated a Chikungunya (Chik) virus from a patient who returned to Germany after a three-month visit to Mauritius in spring 2006. Upon return she developed a transient fever up to 40 degrees C. This was followed by myalgia and joint pain. IgG antibodies in serum to Chik virus were undetectable. Virus (Chik-Wu1) was isolated on Vero cells. We molecularly cloned the whole genome of Chik-Wu1 from viral RNA by RT-PCR. The complete sequence was determined and functional domains of the genome were assigned. Chik-Wu1 clearly belongs to the group of viruses analysed from the recent Indian Ocean outbreak. In order to develop tools useful for further characterization of Chik-Wu1 we bacterially expressed and purified the capsid (C) and envelope (E) proteins and established an immunoblot assay. Twenty-two of 30 serum samples from Chik virus-infected patients that scored positive in indirect immunofluorescence previously were also reactive in immunoblot analysis with recombinant C and E2 antigens.