Nanobodies as novel agents for disease diagnosis and therapy.
Nanobodies as novel agents for disease diagnosis and therapy.
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DOI：
10.2147/ijn.s39428
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发表时间：
2013
影响因子：
8
通讯作者：
Siontorou CG
中科院分区：
文献类型：
--
作者：
Siontorou CG
骆驼科中天然存在的仅重链抗体的发现及其进一步发展为小的重组纳米抗体，在药物递送和成像方面提供了有吸引力的替代方案。纳米抗体衍生物易于在微生物中表达并易于工程化，是可溶的、稳定的、通用的，并且具有独特的重折叠能力、降低的聚集趋势和高靶点结合能力。本文综述了纳米抗体的研究现状，重点介绍了纳米抗体的结构特征和性质、生产、技术以及调节免疫功能和靶向肿瘤、毒素和微生物的潜力。
The discovery of naturally occurring, heavy-chain only antibodies in Camelidae, and their further development into small recombinant nanobodies, presents attractive alternatives in drug delivery and imaging. Easily expressed in microorganisms and amenable to engineering, nanobody derivatives are soluble, stable, versatile, and have unique refolding capacities, reduced aggregation tendencies, and high-target binding capabilities. This review outlines the current state of the art in nanobodies, focusing on their structural features and properties, production, technology, and the potential for modulating immune functions and for targeting tumors, toxins, and microbes.