Labeling thiols on proteins, living cells, and tissues with enhanced emission induced by FRET.
Labeling thiols on proteins, living cells, and tissues with enhanced emission induced by FRET.
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利用 FRET 诱导的增强发射在蛋白质、活细胞和组织上标记硫醇
DOI：
10.1038/srep03523
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发表时间：
2013-12-17
影响因子：
4.6
通讯作者：
Liang, Gaolin
中科院分区：
文献类型：
--
作者：
Yuan, Yue;Wang, Xijun;Mei, Bin;Zhang, Dongxin;Tang, Anming;An, Linna;He, Xiaoxiao;Jiang, Jun;Liang, Gaolin
Using N-(2-Aminoethyl)maleimide-cysteine(StBu) (Mal-Cys) as a medium, protein thiols were converted into N-terminal cysteines. After a biocompatible condensation reaction between the N-terminal cysteine and fluorescent probe 2-cyanobenzothiazole-Gly-Gly-Gly-fluorescein isothiocyanate (CBT-GGG-FITC), a new fluorogenic structure Luciferin-GGG-FITC was obtained. The latter exhibits near one order of magnitude (7 folds) enhanced fluorescence emission compared to the precursor moiety due to fluorescence resonance energy transfer (FRET) effect between the newly formed luciferin structure and the FITC motif. Theoretical investigations revealed the underlying mechanism that satisfactorily explained the experimental results. With this method, enhanced fluorescence imaging of thiols on proteins, outer membranes of living cells, translocation of membrane proteins, and endothelial cell layers of small arteries was successfully achieved.
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影响因子：
15
作者：
Halo TL;Appelbaum J;Hobert EM;Balkin DM;Schepartz A
通讯作者：
Schepartz A
影响因子：
48
作者：
Chen, I;Howarth, M;Ting, AY
通讯作者：
Ting, AY
影响因子：
15
作者：
Cohen AS;Dubikovskaya EA;Rush JS;Bertozzi CR
通讯作者：
Bertozzi CR
DOI：
10.1039/c0cc01398f
发表时间：
2010-08-21
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Lim S;Escobedo JO;Lowry M;Xu X;Strongin R
通讯作者：
Strongin R
影响因子：
46.9
作者：
Guignet, EG;Hovius, R;Vogel, H
通讯作者：
Vogel, H