Semaphorin 4D inhibits collagen synthesis of rat pulp-derived cells
Semaphorin 4D inhibits collagen synthesis of rat pulp-derived cells
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DOI：
10.1016/j.archoralbio.2007.08.005
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发表时间：
2008-01-01
影响因子：
3
通讯作者：
Wakisaka, Satoshi
中科院分区：
文献类型：
--
作者：
Abe, Makoto;Inagaki, Shinobu;Wakisaka, Satoshi
目的：信号蛋白是一组分泌的膜相关分子，在轴突导航中发挥重要作用。几种脑信号蛋白家族分子在咽弓和牙胚中表达。我们分析了牙胚中膜相关信号蛋白 4D (Sema4D) 的表达，并检查了其在体外调节大鼠切牙牙髓来源细胞分化中的潜在作用。设计：通过原位杂交检查 mRNA 表达。通过腺病毒介导的过表达检查 Sema4D 对大鼠牙髓来源细胞的影响。结果：牙胚上皮细胞和间质细胞在钟早期均表达Sema4D。后来，成牙本质细胞主要表达Sema4D，而上皮表达大大减少。过度表达。 Sema4D 在大鼠门牙牙髓衍生细胞中强烈抑制矿化。在这种抑制之前，mRNA 合成水平上的胶原纤维产生减少。结论：这些结果表明 Sema4D 在牙胚的上皮细胞和间充质细胞中表达。 Sema4D 抑制牙髓源性细胞的胶原蛋白合成，表明它可能对成牙本质细胞分化产生负调节。 (C) 2007 Elsevier Ltd. 保留所有权利。
Objective: Semaphorins are a group of secreted and membrane-associated molecules that play important roles in axon navigation. Several semaphorin family molecules are expressed in the pharyngeal arches and tooth germs. We analysed the expression of membrane-associated Semaphorin 4D (Sema4D) in tooth germs, and examined its potential role in regulating the differentiation of rat incisor pulp-derived cells in vitro.Design: mRNA expression was examined by in situ hybridisation. The effects of Sema4D on rat pulp-derived cells were examined by adenovirus-mediated overexpression. in vitro.Results: Both epithelial and mesenchymal cells of tooth germs expressed Sema4D at the early bell stage. Later, the odontoblasts predominantly expressed Sema4D, while the epithelial expression greatly decreased. The overexpression. of Sema4D in rat incisor pulp-derived cells strongly inhibited mineralisation. This inhibition was preceded by a reduction of collagen fibre production at the level of mRNA synthesis.Conclusions: These results indicate that Sema4D is expressed in both epithelial and mesenchymal cells of the tooth germs. Sema4D represses Collagen synthesis of pulp-derived cells, indicating it might negatively regulate odontoblast differentiation. (C) 2007 Elsevier Ltd. All rights reserved.