Association between polymorphisms in FOXP3 and EBI3 genes and the risk for development of allergic rhinitis in Chinese subjects
Association between polymorphisms in FOXP3 and EBI3 genes and the risk for development of allergic rhinitis in Chinese subjects
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FOXP3和EBI3基因多态性与中国受试者发生过敏性鼻炎的风险之间的关联
DOI：
10.1016/j.humimm.2012.07.319
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发表时间：
2012-09-01
期刊：
影响因子：
2.7
通讯作者：
Zhang, Luo
中科院分区：
文献类型：
--
作者：
Zhang, Yuan;Duan, Su;Zhang, Luo
目的：探讨叉头盒蛋白3（FOXP3）和EBV诱导基因3（EBI3）基因多态性是否与中国患者变应性鼻炎（AR）相关。方法：采用基于人群的病例对照关联研究设计评估FOXP3和EBI3基因区域单核苷酸多态性（SNP）所带来的AR风险。从 378 名 AR 患者和 330 名健康对照中提取 DNA，并分析选定和标记的 SNP。总共选择了9个SNP并进行了基因分型。结果：在AR风险的单位点分析中，EBI3基因中rs428253的等位基因频率在AR患者和对照受试者之间存在显着差异（P = 1.00E-04）；即使经过 10,000 次排列（P < 0.05）。根据年龄和性别进行调整后的 Logistic 回归分析进一步显示 EBI3 rs428253 与 AR 保护作用之间存在显着相关性（对于 CG/CC，P = 0.015，OR = 0.624）。 FOXP3 基因中带有“AG”的双倍型 rs3761548-rs4824747 与 AR 风险相关（P = 0.031，OR = 1.755）。结论：本研究的结果支持调节性 T 细胞以及 FOXP3 和 EBI3 基因周围区域的遗传变异在改变中国患者 AR 发生风险中的潜在作用。 (C) 2012 年美国组织相容性和免疫遗传学学会。由爱思唯尔公司出版。保留所有权利。
Objective: To investigate whether polymorphisms in forkhead box protein 3 (FOXP3) and EBV-induced gene 3 (EBI3) genes are associated with allergic rhinitis (AR) in Chinese patients.Methods: A population-based case-control association study design was used to assess the risk of AR conferred by by single nucleotide polymorphisms (SNPs) in FOXP3 and EBI3 gene regions. DNA was extracted from 378 patients with AR and 330 healthy controls and analyzed for selected and tagged SNPs. Overall, 9 SNPs were selected and genotyped.Results: In the single-locus analyses of AR risk, the allele frequencies of rs428253 in EBI3 gene were significantly different between the AR patients and control subjects (P = 1.00E-04); even after 10,000 permutations (P < 0.05). Logistic regression analyses, adjusted for age and gender, further showed a significant association between EBI3 rs428253 and protective effects against AR (P = 0.015, OR = 0.624 for CG/CC). The diplotype rs3761548-rs4824747 in FOXP3 gene with "AG" was associated with risk of AR (P= 0.031, OR = 1.755).Conclusions: The findings of this study support the potential role of regulatory T cells and genetic variations in the regions around FOXP3 and EBI3 genes in modifying the risk for AR development in Chinese patients. (C) 2012 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.