Investigations of Soret excited resonance Raman excitation profiles in cytochrome c
Investigations of Soret excited resonance Raman excitation profiles in cytochrome c
复制标题
细胞色素 c 中 Soret 激发共振拉曼激发谱的研究
DOI：
10.1063/1.438455
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发表时间：
1979
影响因子：
4.4
通讯作者：
A. C. Albrecht
中科院分区：
文献类型：
--
作者：
P. Champion;A. C. Albrecht
使用Soret区域（350-450 nm）的可调谐激光激发获得铁-和亚铁细胞色素c中“氧化态标记带”的共振拉曼激发曲线。激发分布是不对称的，峰值明显的Soret吸收最大值的蓝色。数据解释使用一个简单的模型，分析包括完整的子空间的弗兰克-康登主动振动与每个中间电子状态。在这个简单的多模模型中，阻尼因子是一个与波长无关的“软”拟合参数。然而，实现了Soret吸收带和拉曼激发分布的极好拟合。我们的理论方法将Soret阻尼因子的上限设置为铁细胞色素c的50 cm−1（HWHM）数量级和铁细胞色素c的300 cm−1（HWHM）数量级。
Resonance Raman excitation profiles of the ’’oxidation state marker bands’’ in ferric‐ and ferrocytochrome c are obtained using tunable laser excitation in the Soret region (350–450 nm). The excitation profiles are asymmetric and peak distinctively to the blue of the Soret absorption maximum. The data are explained using a simple model that analytically includes the complete subspace of Franck–Condon active vibrations associated with each intermediate electronic state. In this simple multimode model the damping factor is a ’’soft’’ fitting parameter taken to be wavelength independent. Nevertheless, an excellent fit of the Soret absorption band and Raman excitation profile is achieved. Our theoretical approach places upper limits on the Soret damping factors that are on the order of 50 cm−1 (HWHM) for ferrocytochrome c and on the order of 300 cm−1 (HWHM) for ferricytochrome c.