Clinicopathological associations of CD44 mRNA and protein expression in primary breast carcinomas
Clinicopathological associations of CD44 mRNA and protein expression in primary breast carcinomas
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DOI：
10.1046/j.1365-2559.2003.01622.x
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发表时间：
2003-06-01
期刊：
影响因子：
6.4
通讯作者：
Nesland, JM
中科院分区：
文献类型：
--
作者：
Berner, HS;Suo, Z;Nesland, JM
目的：本研究的目的是研究CD 44亚型在乳腺癌的发生和预测临床outcome.Methods和结果的作用：休克冷冻肿瘤组织从110例乳腺癌进行了检查，免疫组化使用针对CD 44 s，V5，V6，V7和V3-10的抗体。此外，这些肿瘤中的80例可用于CD 44 s和CD 44 v6的定量实时逆转录酶-聚合酶链反应（RT-PCR）分析。免疫组化，阳性肿瘤细胞质和/或膜染色与所有抗体。染色结果与组织学亚型、淋巴结状态、类固醇受体状态、肿瘤大小或年龄无关。总体生存率和无病生存率也没有发现任何相关性。然而，CD 44 s和CD 44 v6的定量实时RT-PCR显示，CD 44 v6 mRNA的表达与较低的病理分级显著相关（Pearson χ 2检验P=0.009;线性-线性相关P=0.003）。CD 44 s mRNA表达与低病理分级之间也存在线性相关性（P=0.02）。Kaplan-Meier法生存分析显示，CD 44 s mRNA表达增加与无病生存期和总生存期显著相关（分别为P=0.0185和P=0.0344）。CD 44 v6 mRNA表达的趋势相似，在这些情况下，但差异不显着。结论：定量实时RT-PCR显示临床相关性的CD 44 s和CD 44 v6 mRNA表达在乳腺癌，而免疫组化的CD 44 s和其他CD 44变体的蛋白表达没有。这一矛盾的结果值得进一步研究CD 44分子在乳腺癌中的临床影响。
Aims: The purpose of this study was to examine the occurrence of CD44 isoforms in breast carcinomas and their role in predicting clinical outcome.Methods and results: Shock-frozen tumour tissues from 110 patients with breast carcinoma were examined by immunohistochemistry using antibodies directed against CD44s, v5, v6, v7 and v3-10. In addition, 80 of these tumours were available for quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of CD44s and CD44v6. Immunohistochemically, the positive tumours showed cytoplasmic and/or membranous staining with all antibodies. Staining results did not correlate with histological subtype, lymph node status, status of steroid receptors, tumour size or age. Neither was any correlation found for overall and disease-free survival. Quantitative real-time RT-PCR of CD44s and CD44v6, however, revealed that expression of CD44v6 mRNA was significantly associated with lower pathological grade (Pearson chi(2) test P=0.009; linear-by-linear association P=0.003). Linear-by-linear association between CD44s mRNA expression and lower pathological grade was also seen (P=0.02). Survival analysis with the Kaplan-Meier method demonstrated that increased CD44s mRNA expression was significantly associated with both disease-free survival and overall survival (P=0.0185 and P=0.0344, respectively). A similar trend for CD44v6 mRNA expression was seen in these cases, but the difference was not significant.Conclusions: Quantitative real-time RT-PCR revealed clinical correlations of CD44s and CD44v6 mRNA expression in breast carcinomas while immunohistochemistry for the protein expression of CD44s and other CD44 variants did not. This contradictory result merits further studies concerning the clinical impact of CD44 molecules in breast carcinomas.