An integrated microfluidic system for long-term perfusion culture and on-line monitoring of intestinal tissue models
An integrated microfluidic system for long-term perfusion culture and on-line monitoring of intestinal tissue models
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DOI：
10.1039/b717091b
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发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Fujii, Teruo
中科院分区：
文献类型：
--
作者：
Kimura, Hiroshi;Yamamoto, Takatoki;Fujii, Teruo
生命科学和医学应用中传统的基于细胞的检测可能难以长期维持功能。微流控是一项新兴技术，有潜力为细胞维护、连续灌注和监测提供集成环境。在这项研究中，我们开发了一种具有片上泵送和检测功能的集成微流体装置。装置中的微流体结构被分成两个独立的通道，由半透膜隔开，在通道上接种和培养细胞。每个通道的培养基的灌注和荧光测量可以通过片上泵系统和光纤检测系统进行。通过长期培养和监测肠道组织模型（Caco-2 细胞）的极化运输活动来检查该装置的性能。细胞可以培养两周以上，并且通过在线荧光测量成功监测了罗丹明123的单层转运。该装置可应用于毒性测试和药物筛选。
Conventional cell-based assays in life science and medical applications can be difficult to maintain functionally over long periods. Microfluidics is an emerging technology with potential to provide integrated environments for cell maintenance, continuous perfusion, and monitoring. In this study, we developed an integrated microfluidic device with on-chip pumping and detection functionalities. The microfluidic structure in the device is divided into two independent channels separated by a semipermeable membrane on which cells are inoculated and cultured. Perfusion and fluorescence measurements of culture media for each channel can be conducted by the on-chip pumping system and optical fiber detection system. Performance of the device was examined through long-term culture and monitoring of polarized transport activity of intestinal tissue models (Caco-2 cells). The cells could be cultured for more than two weeks, and monolayer transport of rhodamine 123 was successfully monitored by on-line fluorescent measurement. This device may have applications in toxicity testing and drug screening.