Generation of non-integrated induced pluripotent stem cells from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome
Generation of non-integrated induced pluripotent stem cells from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome
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DOI：
10.1016/j.scr.2016.12.002
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发表时间：
2017-01-01
影响因子：
1.2
通讯作者：
Li, Yin-xiong
中科院分区：
文献类型：
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作者：
Guo, Dongsheng;Wu, Feima;Li, Yin-xiong
尿源细胞取自一名23岁的男性多发性内分泌瘤1型综合征(MEN1)患者，用于产生带有上体质粒的iPS细胞。建立了两个稳定的不含表型质粒的IPSC系。该患者在MEN1基因外显子9上存在杂合性G-N-T突变，通过对所有得到的细胞系进行测序分析证实。核型分析显示染色体形态和数目正常。通过基因表达和分化为三个生殖层的能力证明了它们的多能性。这些IPSC系为MEN1综合征的病理学研究提供了有价值的体外资源。(C)2016年提交人。爱思唯尔出版公司(Elsevier B.V.)
Urine resource cellswere collected froma 23-year-oldmalewithmultiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cellswith episomal plasmids. Two stable iPSC lineswith free of episomal plasmidwere established. The patient has a heterozygous G N T mutation on the exon 9 of Men1 gene that was confirmed by sequencing analysis on all resulted cell lines. Karyotyping indicated the chromosomes with normal appearances and numbers. Their pluripotencywas demonstrated by gene expression and their abilities for differentiating into three germlayers. These iPSC lines provide valuable in vitro resources for pathological study on MEN1 syndrome. (C) 2016 The Authors. Published by Elsevier B.V.