The rice aldehyde oxidase OsAO3 gene regulates plant growth, grain yield, and drought tolerance by participating in ABA biosynthesis
The rice aldehyde oxidase OsAO3 gene regulates plant growth, grain yield, and drought tolerance by participating in ABA biosynthesis
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水稻醛氧化酶 OsAO3 基因通过参与 ABA 生物合成来调节植物生长、谷物产量和耐旱性。
DOI：
10.1016/j.bbrc.2021.02.047
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发表时间：
2021-02-26
影响因子：
3.1
通讯作者：
Jing, Wen
中科院分区：
文献类型：
--
作者：
Shi, Xingyu;Tian, Quanxiang;Jing, Wen
脱落酸 (ABA) 调节植物生长和发育的许多方面以及对非生物胁迫的反应。拟南芥醛氧化酶 3 (AAO3) 催化 ABA 生物合成的最后一步。我们克隆了一种新的醛氧化酶基因 OsAO3，并对其进行了功能表征，OsAO3 是 AAO3 的水稻同源物。 OsAO3在发芽种子、根、叶和花器官中表达，特别是在维管组织和保卫细胞中，并且其表达被外源ABA和甘露醇显着诱导。在正常和干旱胁迫条件下，OsAO3 的突变和过度表达分别降低和增加了幼苗芽和根中的 ABA 水平。与野生型相比，osao3 突变体表现出更早的种子发芽、加速的幼苗生长和降低的耐旱性，OsAO3 过表达品系则表现出相反的表型。 OsAO3 的突变和过度表达分别通过影响每株植物的穗数、每穗的小穗数和小穗育性来增加和降低谷物产量。因此，OsAO3 可能参与 ABA 生物合成，对于调节水稻种子发芽、幼苗生长、籽粒产量和耐旱性至关重要。(c) 2021 Elsevier Inc. 保留所有权利。
Abscisic acid (ABA) regulates many aspects of plant growth and development and the responses to abiotic stresses. Arabidopsis aldehyde oxidase 3 (AAO3) catalyzes the final step of ABA biosynthesis. We cloned and functionally characterized a novel aldehyde oxidase gene, OsAO3, the rice homolog of AAO3. OsAO3 was expressed in germinated seeds, roots, leaves, and floral organs, particularly in vascular tissues and guard cells, and its expression was significantly induced by exogenous ABA and mannitol. Mutation and overexpression of OsAO3 decreased and increased ABA levels, respectively, in seedling shoots and roots under both normal and drought stress conditions. The osao3 mutant exhibited earlier seed germination, increased seedling growth, and decreased drought tolerance compared to the wild-type, OsAO3-overexpressing lines exhibited the opposite phenotype. Mutation and overexpression of OsAO3 increased and decreased grain yield, respectively, by affecting panicle number per plant, spikelet number per panicle, and spikelet fertility. Thus, OsAO3 may participate in ABA biosynthesis, and is essential for regulation of seed germination, seedling growth, grain yield, and drought tolerance in rice.(c) 2021 Elsevier Inc. All rights reserved.