Interaction of leptin and nitric oxide pathway on penicillin-induced epileptiform activity in rats
Interaction of leptin and nitric oxide pathway on penicillin-induced epileptiform activity in rats
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DOI：
10.1016/j.brainres.2010.01.054
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发表时间：
2010-03-19
期刊：
影响因子：
2.9
通讯作者：
Agar, Erdal
中科院分区：
文献类型：
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作者：
Aslan, Ali;Yildirim, Mehmet;Agar, Erdal
本研究的目的是探讨NO作为瘦素作用的介质在青霉素诱导的大鼠癫痫样活动中的作用。青霉素注射后30分钟，瘦素，在剂量为1钻机，显着增加癫痫样活动的平均频率，而不改变振幅。观察了全身应用一氧化氮合酶（NOS）抑制剂L-精氨酸甲酯（L-NAME）、选择性神经元型NOS抑制剂7-硝基吲唑（7-NI）和NO前体L-精氨酸对瘦素作用的影响。7-NI（40 mg/kg，i.p.）在瘦素（1 μ g）存在下显著延迟。腹腔注射L-NAME（60 mg/kg），30毫米前瘦素（1 μ g）的应用，不影响促惊厥活性的瘦素。L-精氨酸（1000 mg/kg，i. p.）在有效剂量的瘦素（1 μ g，i. c. v.）逆转瘦素的促惊厥作用，而相同剂量的其无活性对映体D-精氨酸（1000 mg/kg，i. p.）未能影响瘦素的促惊厥作用。本研究的电生理证据表明，神经元NOS/NO通路参与介导瘦素对青霉素诱导的癫痫样活动的影响。(C)2010 Elsevier B. V.保留所有权利。
The aim of the present study was to investigate the role of NO as a mediator of leptin action at the penicillin-induced epileptiform activity in rat. Thirty minutes after penicillin injection, leptin, at a dose of 1 rig, significantly increased the mean frequency of epileptiform activity without changing the amplitude. The effects of systemic administration of nitric oxide synthase (NOS) inhibitors, non-selective NG-nitro-L-arginine methyl ester (L-NAME), selective neuronal NOS inhibitor, 7-nitroindazole (7-NI) and NO precursor, L-arginine on the effects of leptin were investigated. The occurrence of anticonvulsant activity of 7-NI (40 mg/kg, i.p.) was significantly delayed in the presence of leptin (1 mu g). The administration of L-NAME (60 mg/kg, i.p.), 30 mm before leptin (1 mu g) application, did not influence proconvulsant activity of leptin. The administration of L-arginine (1000 mg/kg, i.p.) 30 min before the effective dose of leptin (1 mu g, i.c.v.) reversed the proconvulsant effects of leptin whereas the same dose of its inactive enantiomer, D-arginine (1000 mg/kg, i.p.) failed to influence the proconvulsant effect of leptin. The electrophysiological evidence of the present study suggests that neuronal NOS/NO pathway is involved in mediating leptin effects on penicillin-induced epileptiform activity. (C) 2010 Elsevier B.V. All rights reserved.