Incorporation and metabolic conversion of erucic acid in various tissues of the rat in short term experiments
Incorporation and metabolic conversion of erucic acid in various tissues of the rat in short term experiments
复制标题
短期实验中芥酸在大鼠各组织中的掺入和代谢转化
作者：
N. Ong;J. Bézard;J. Lecerf
给大鼠静脉注射游离(14-14C)芥酸(22∶1)和(9-103H)油酸(18∶1)的混合物。 2、4、8、16和30分钟后，检查血液、肝脏、心脏、肾脏和脾脏的放射性。在 0 至 8 分钟内，游离 (14C) 22:1 从血液中消失的速度比游离 (14C) 18:1 更快。标记掺入甘油三酯仅在 16 分钟后出现，在 30 分钟时它们占注射放射性的 4%。在该级分中，63%的14C放射性以18∶1存在，而不是原来的22∶1，而几乎所有3H放射性恢复为未改变的18∶1。在所有研究时间中，大部分放射性都在肝脏中发现，主要以甘油三酯（总脂质中放射性的 60%）和磷脂（20-30%）的形式存在。 14C 的存在比例几乎与 3H 相同（仅 2 分钟后注射放射性的 13%，30 分钟时为 11%）。 14C 放射性在 18:1 中的比例高于 22:1（45%）。甘油三酯，65% 为磷脂）。由于血液中富含(14C)18∶1的标记甘油三酯主要来源于肝脏甘油三酯，因此看来18∶1是22∶1在肝脏转化后在组织中利用的主要形式。在其他测试器官中，发现放射性比肝脏低 10-15 倍。在心脏中，14C比3H高3至4倍。 80%以上以22:1的甘油三酯形式回收。在脾脏和肾脏中，游离脂肪酸和单酸甘油酯中的 14 C: 3 H 比率特别高。在肾脏中，60%的14 C以神经酸(24∶1)的形式存在于甘油单酯中，40%以磷脂的形式存在，表明形成的单神经酸用于磷脂的生物合成。
Rats were intravenously injected with a mixture of free (14-14C) erucic acid (22∶1) and (9–103H) oleic acid (18∶1). After 2, 4, 8, 16, and 30 min, radioactivity was examined in blood, liver, heart, kidneys, and spleen. Free (14C) 22∶1 disappeared from the blood more rapidly than free (14C) 18∶1 between 0 and 8 min. Incorporation of label into triglycerides only appeared after 16 min and at 30 min they represented 4% of the injected radioactivity. In this fraction, 63% of14C radioactivity was present as 18∶1 and not as the original 22∶1, while almost all3H radioactivity was recovered as unchanged 18∶1. At all times studied, the majority of radioactivity was found in the liver, primarily as triglycerides (60% of radioactivity in total lipids) and as phospholipids (20–30%).14C was present in nearly the same proportion as3H (13% of injected radioactivity after only 2 min, 11% at 30 min).14C radioactivity was contained in 18∶1 in higher proportion than 22∶1 (45% in triglycerides, 65% in phospholipids). Since labeled triglycerides of blood, rich in (14C) 18∶1, mainly originate from the liver triglycerides, it appears that 18∶1 is the major form of utilization of 22∶1 in the tissues after its conversion in liver. In the other organs tested, radioactivity was found 10–15 times lower than in liver. In the heart,14C was 3 to 4 times higher than3H. More than 80% was recovered as 22∶1 in triglycerides. In spleen and kidneys, the14C:3H ratio was particularly high in free fatty acids and monoglycerides. In kidneys, 60% of14C was present as nervonic acid (24∶1) in monoglycerides and 40% in phospholipids, suggesting that the mononervonin formed was used for phospholipid biosynthesis.