Dental microwear texture analysis of extant sika deer with considerations on inter-microscope variability and surface preparation protocols.
Dental microwear texture analysis of extant sika deer with considerations on inter-microscope variability and surface preparation protocols.
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对现存梅花鹿的牙齿微磨损纹理分析，考虑显微镜间变异性和表面处理方案。
DOI：
10.1016/j.bsbt.2017.11.006
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发表时间：
2017
影响因子：
--
通讯作者：
T. and Kohno N.
中科院分区：
文献类型：
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作者：
Kubo;M.O.;Yamada;E.;Kubo;T. and Kohno N.
近年来，牙齿微磨损纹理分析（DMTA）已被应用于各种脊椎动物的饮食偏好推断。需要更多关于显微镜间变异性的数据，以确保该方法的客观性和可重复性。在本论文中，我们研究了两个共聚焦激光显微镜之间的显微镜变异性与不同的规格，以及由于不同的协议应用到DMTA之前获得的表面的变异性。我们使用了两种不同的DMTA方法：尺度敏感分形分析（SSFA）和表面纹理分析（STA）。我们收集了现存的日本西卡鹿种群的DMTA数据与不同的饮食。我们发现，不同的协议产生显着不同的结果SSFA和STA，而近三分之二的参数没有显着不同的机器时，应用相同的预分析协议。最后，我们分析了来自11个种群的244只西卡的DMTA数据。29个参数在人群中存在显著差异。放牧西卡鹿的牙釉质表面主要是一些排列良好的划痕，而浏览鹿的牙釉质表面更平坦，其特征是更零星的坑。因此，DMTA可以识别种内的饮食变异，这是小于现存反刍动物种间的饮食变异。
Recently, dental microwear texture analysis (DMTA) has been applied to various kinds of vertebrates to infer dietary preference. More data are needed on inter-microscope variability to assure the objectivity and repeatability of this method. In the present paper, we investigated inter-microscope variability between two confocal laser microscopes with different specifications, as well as variability due to different protocols applied to the obtained surface before DMTA. We used two different methods of DMTA: the scale sensitive fractal analysis (SSFA) and the surface texture analysis (STA). We collected DMTA data of extant Japanese sika deer populations with varying diets. We found that different protocols produced significantly different results for both SSFA and STA, whereas nearly two thirds of the parameters were not significantly different between the machines when applying the same pre-analysis protocol. Finally, we analyzed DMTA data of 244 sika deer from 11 populations. Twenty nine parameters were significantly different among the populations. Tooth enamel surface of grazing sika deer is dominated by a number of well-aligned scratches, whereas that of browsing deer is more flattened and characterized by more sporadic pits. Therefore, DMTA can identify intraspecific variation in diets, which is smaller than the interspecific dietary variation in extant ruminants.