Protein deformation of lipid hybrid bilayer membranes studied by sum frequency generation vibrational spectroscopy
Protein deformation of lipid hybrid bilayer membranes studied by sum frequency generation vibrational spectroscopy
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DOI：
10.1021/la0484220
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发表时间：
2004-10-12
期刊：
影响因子：
3.9
通讯作者：
Neivandt, DJ
中科院分区：
文献类型：
--
作者：
Doyle, AW;Fick, J;Neivandt, DJ
本文首次利用和频产生振动光谱的固有表面特异性非线性光学技术研究了无信号肽（SPL）蛋白诱导的脂质杂化双层膜的结构变形。具体来说，1,2-二硬脂酰磷脂酰甘油（DSPG）膜的变形是由FGF-1相互作用诱导的，FGF-1是一种spg蛋白，作为细胞应激的一个功能，通过非经典途径释放。研究发现，FGF-1在60℃下的极低浓度（1 nM）下可诱导先前高度有序的DSPG膜中的脂质烷基链变形。在用缓冲溶液冲洗去除蛋白质后，变形过程显示出一定程度的可逆性。
Structural deformations of lipid hybrid bilayer membranes induced by signal peptideless (SPL) proteins have been studied for the first time using the inherently surface specific nonlinear optical technique of sum frequency generation vibrational spectroscopy. Specifically, deformations of 1,2-distearoylphosphatidylglycerol (DSPG) membranes induced by interaction with FGF-1, a SPL protein which is released as a function of cellular stress through a nonclassical pathway, have been investigated. FGF-1 was found to induce lipid alkyl chain deformations in previously highly ordered DSPG membranes at the extremely low concentration of 1 nM at 60degreesC. The deformation process was shown to exhibit a degree of reversibility upon removal of the protein by rinsing with buffer solution.