Development of 21 microsatellite primers for Camellia pingguoensis (Theaceae) using 454 sequencing
Development of 21 microsatellite primers for Camellia pingguoensis (Theaceae) using 454 sequencing
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利用454测序开发平果茶（山茶科）21个微卫星引物
DOI：
10.1007/s12686-014-0221-9
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发表时间：
2014-09-01
影响因子：
1.1
通讯作者：
Tang, Shao-Qing
中科院分区：
文献类型：
--
作者：
Lu, Yong-Bin;Liufu, Yong-Qing;Tang, Shao-Qing
苹果茶是一种濒危、名贵的观赏植物。在这里，我们使用 454 测序分离并表征了平果 C. pingguoensis 的 21 个多态性微卫星位点。这些标记在平果 C. pingguensis var. 的一个种群上进行了测试。 Terminalis 和密切相关的 C. impressinevis 的一个种群。对于平果线虫来说，所有这些位点均表现出多态性，每个位点的等位基因数量为3至9个。观察到的和预期的杂合度分别为0.200至0.963和0.246至0.868。这些标记对于平果虫种群遗传结构、遗传多样性和规划保护管理的研究具有重要意义。这些基因座也可用于其他相关的山茶花物种。
Camellia pingguoensis is an endangered and valuable ornamental plant species. Here we isolated and characterized 21 polymorphic microsatellite loci for C. pingguoensis using 454 sequencing. The markers were tested on one population of C. pingguoensis var. terminalis and one population of closely related C. impressinevis. For C. pingguoensis, all of these loci showed polymorphism, and the number of alleles per locus ranged from 3 to 9. The observed and expected heterozygosities ranged from 0.200 to 0.963 and from 0.246 to 0.868, respectively. These markers will be useful in the research on the population genetic structure, genetic diversity and planning conservation management of C. pingguoensis. These loci can also be used for other related Camellia species.