Detection of immunoglobulin M antibodies to hepatitis E virus by class capture enzyme immunoassay.
Detection of immunoglobulin M antibodies to hepatitis E virus by class capture enzyme immunoassay.
复制标题
通过类捕获酶免疫分析检测戊型肝炎病毒的免疫球蛋白 M 抗体。
DOI：
10.1128/cdli.10.4.579-586.2003
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Purcell,RH
中科院分区：
文献类型：
--
作者：
Yu,C;Engle,RE;Bryan,JP;Emerson,SU;Purcell,RH
戊型肝炎病毒抗体(抗-HEV)的测定对于了解戊型肝炎的流行病学至关重要。要确定HEV感染的流行率，需要一种可靠的、灵敏和特异的血清学检测。区分感染的急性期和恢复期也很重要；这通常需要检测免疫球蛋白M(IgM)类抗体。目前已报道的检测IgM抗HEV抗体的酶免疫分析(EIA)方法很少，大多数采用夹心法。本研究描述了一种通过抗体类别捕获方法检测IgM抗-HEV的EIA。该检测是通过使用实验感染的非人类灵长类动物的血清和/或血浆面板进行验证的。它被用来显示记忆反应和IgM抗-HEV在两个不同的时间间隔45个月的黑猩猩实验中的再现。在检测巴基斯坦两起戊型肝炎疫情的临床标本时，类别捕捉法比夹心EIA法更灵敏；在存在高滴度的抗-HEV的情况下，它也具有区分IgM-抗-HEV的优势。
The measurement of antibodies to hepatitis E virus (anti-HEV) has been essential for understanding the epidemiology of hepatitis E. Studies to determine the prevalence of HEV infections require a reliable serologic assay that is sensitive and specific. It is also important to distinguish the acute from the convalescent phase of an infection; this usually requires the detection of the immunoglobulin M (IgM) class of antibody. Few enzyme immunoassays (EIAs) that measure IgM anti-HEV have been described, and most have utilized the sandwich method. The present study describes an EIA that detects IgM anti-HEV by antibody class capture methodology. The assay was validated by using serum and/or plasma panels from experimentally infected nonhuman primates. It was used to demonstrate an anamnestic response and the reappearance of IgM anti-HEV in a chimpanzee experimentally challenged with HEV at two different times 45 months apart. The class capture method was more sensitive than the sandwich EIA when used to test clinical samples from two hepatitis E epidemics in Pakistan; it also had the advantage of distinguishing IgM anti-HEV in the presence of high titers of IgG anti-HEV.