Nitrogen doped graphene nanosheet supported platinum nanoparticles as high performance electrochemical homocysteine biosensors
Nitrogen doped graphene nanosheet supported platinum nanoparticles as high performance electrochemical homocysteine biosensors
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DOI：
10.1039/c3tb20923g
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发表时间：
2013-01-01
影响因子：
7
通讯作者：
Opallo, Marcin
中科院分区：
文献类型：
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作者：
Kannan, Palanisamy;Maiyalagan, Thandavarayan;Opallo, Marcin
功能性碳纳米材料对于开发高性能、高灵敏度和高选择性的电化学生物传感器具有重要意义。本研究采用简单的化学还原法合成了石墨烯负载铂纳米颗粒（GN-PtNPs）和氮掺杂石墨烯负载铂纳米颗粒（N-GN-PtNPs），并首次将其作为高性能纳米催化剂载体和掺杂纳米催化剂载体用于同型半胱氨酸（HCY）的电化学氧化。我们的研究表明，N掺杂的石墨烯负载的PtNPs显示出更高的HCY的电催化活性，实验检测限为200 pM。此外，N掺杂的石墨烯负载的Pt被证明在HCY的电化学氧化中具有优异的选择性，即，HCY的检测在20倍过量的抗坏血酸（AA）存在下是成功的。N-掺杂的石墨烯负载的PtNP材料的实际应用有效地显示了在优化条件下通过差分脉冲伏安法测定人血清和尿液样品中的HCY。我们的研究结果得出结论，N掺杂的石墨烯负载的PtNPs可以被开发为用于电化学生物传感器应用的高性能和多功能的纳米电催化剂。
Functional carbon nanomaterials are significantly important for the development of high performance sensitive and selective electrochemical biosensors. In this study, graphene supported platinum nanoparticles (GN-PtNPs) and nitrogen doped graphene supported platinum nanoparticles (N-GN-PtNPs) were synthesized by a simple chemical reduction method and explored as high performance nanocatalyst supports, as well as doped nanocatalyst supports, toward electrochemical oxidation of homocysteine (HCY) for the first the time. Our studies demonstrate that N-doped graphene supported PtNPs show higher electrocatalytic activity for HCY with an experimental detection limit of 200 pM. Moreover, N-doped graphene supported Pt was demonstrated to have excellent selectivity in the electrochemical oxidation of HCY i.e., the detection of HCY is successful in the presence of a 20-fold excess of ascorbic acid (AA). The practical application of N-doped graphene supported PtNP materials is effectively shown for the determination of HCY in both human blood serum and urine samples, by differential pulse voltammetry under optimized conditions. Our findings conclude that N-doped graphene supported PtNPs can be developed as a high performance and versatile nano-electrocatalyst for electrochemical biosensor applications.