Hepatoprotective and hypolipidaemic effects of glycoprotein isolated from Gardenia jasminoides Ellis in mice
Hepatoprotective and hypolipidaemic effects of glycoprotein isolated from Gardenia jasminoides Ellis in mice
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DOI：
10.1111/j.1440-1681.2006.04466.x
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发表时间：
2006-10-01
影响因子：
2.9
通讯作者：
Lim, Kye-Taek
中科院分区：
文献类型：
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作者：
Lee, Sei-Jung;Oh, Phil-Sun;Lim, Kye-Taek
本研究旨在研究栀子中一种分子量为27 kDa的糖蛋白的保肝降血脂作用在葡萄糖/葡萄糖氧化酶（G/GO）处理的BNL CL. 2细胞中，以及在CCl 4、Triton WR-1339和玉米油处理的小鼠中，结果表明，GJE糖蛋白对G/GO诱导的细胞毒性和细胞内活性氧的产生具有抑制作用。此外，GJE糖蛋白对Fe ~（2+）/抗坏血酸体系中脂质过氧化过程具有抗氧化作用，在CCl_4（1.0 mL/kg）处理的小鼠中，GJE糖蛋白（80 mg/kg）可阻断乳酸脱氢酶的释放和硫代巴比妥酸反应性物质的形成。此外，在这些小鼠中，GJE导致一氧化氮产生增加和抗氧化酶的激活，伴随着细胞毒性相关信号肝细胞色素c、核因子-κ B和激活蛋白-1的抑制。（400毫克/千克）和玉米油（1.0 g/kg）给药小鼠，GJE糖蛋白预处理（80 mg/kg）降低血浆脂蛋白水平（甘油三酯、总胆固醇和低密度脂蛋白）。基于这些结果，我们假设GJE糖蛋白可以改善肝功能，因为它具有保肝和降血脂活性。
The present study was performed to investigate the hepatoprotective and hypolipidaemic effects of a 27 kDa glycoprotein isolated from Gardenia jasminoides Ellis (GJE glycoprotein) in glucose/glucose oxidase (G/GO)-treated BNL CL.2 cells, as well as in CCl4, Triton WR-1339 and corn oil-treated mice.In G/GO-treated BNL CL.2 cells, the results showed that GJE glycoprotein has an inhibitory effect on G/GO-induced cytotoxicity and intracellular reactive oxygen species production. In addition, GJE glycoprotein has an anti-oxidant effect against the lipid peroxidation process in the Fe2+/ascorbic acid system.In CCl4 (1.0 mL/kg)-treated mice, pretreatment with GJE glycoprotein (80 mg/kg) blocked lactate dehydrogenase release and the formation of thiobarbituric acid-reactive substances. In addition, in these mice GJE resulted in increased nitric oxide production and the activation of anti-oxidant enzymes, accompanied by the inhibition of the cytotoxic-related signals hepatic cytochrome c, nuclear factor-kappa B and activator protein-1.In both Triton WR-1339 (400 mg/kg) and corn oil (1.0 g/kg)-treated mice, pretreatment with GJE glycoprotein (80 mg/kg) lowered the levels of plasma lipoproteins (triglyceride, total cholesterol and low-density lipoprotein).On the basis of these results, we assume that GJE glycoprotein can ameliorate liver function, because it has hepatoprotective and hypolipidaemic activities.