Pleiotropic Roles of Polyglycerolphosphate Synthase of Lipoteichoic Acid in Growth of Staphylococcus aureus Cells
Pleiotropic Roles of Polyglycerolphosphate Synthase of Lipoteichoic Acid in Growth of Staphylococcus aureus Cells
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DOI：
10.1128/jb.01221-08
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发表时间：
2009-01-01
影响因子：
3.2
通讯作者：
Sekimizu, Kazuhisa
中科院分区：
文献类型：
--
作者：
Oku, Yusuke;Kurokawa, Kenji;Sekimizu, Kazuhisa
脂磷壁酸（LTA）是革兰氏阳性菌金黄色葡萄球菌表面的两种阴离子聚合物之一。LTA对于细菌-宿主细胞相互作用是关键的，并且最近已被证明是细胞生长和分裂所需的。为了确定LTA的其他生物学作用，我们发现有必要确定LTA缺陷突变体生长的许可条件。我们发现，一个缺乏LTA的S。金黄色葡萄球菌Δ ltaS突变体可以在30 ℃生长，但不能在37 ℃生长。即使在允许的温度下，Delta ltaS突变细胞也有异常的细胞分裂和分离，自溶减少，肽聚糖水解酶水平降低。Δ ltaS突变细胞升高至非允许温度导致不能排除Sytox绿色染料。高渗透压的生长培养基显着挽救了在37摄氏度下的Delta ltaS突变体的集落形成能力，表明LTA合成是在低渗透压条件下生长所必需的。此外，发现Δ ltaS突变与Δ tagO突变在合成上是致命的，Δ tagO突变在30 ° C下破坏另一种阴离子聚合物壁磷壁酸（WTA）的合成，这表明LTA和WTA在基本功能上相互补偿。
Lipoteichoic acid (LTA) is one of two anionic polymers on the surface of the gram-positive bacterium Staphylococcus aureus. LTA is critical for the bacterium- host cell interaction and has recently been shown to be required for cell growth and division. To determine additional biological roles of LTA, we found it necessary to identify permissive conditions for the growth of an LTA-deficient mutant. We found that an LTA-deficient S. aureus Delta ltaS mutant could grow at 30 degrees C but not at 37 degrees C. Even at the permissive temperature, Delta ltaS mutant cells had aberrant cell division and separation, decreased autolysis, and reduced levels of peptidoglycan hydrolases. Upshift of Delta ltaS mutant cells to a nonpermissive temperature caused an inability to exclude Sytox green dye. A high-osmolarity growth medium remarkably rescued the colony-forming ability of the Delta ltaS mutant at 37 degrees C, indicating that LTA synthesis is required for growth under low-osmolarity conditions. In addition, the Delta ltaS mutation was found to be synthetically lethal with the Delta tagO mutation, which disrupts the synthesis of the other anionic polymer, wall teichoic acid (WTA), at 30 degrees C, suggesting that LTA and WTA compensate for one another in an essential function.