Caenorhabditis elegans as a model host for Staphylococcus aureus pathogenesis
Caenorhabditis elegans as a model host for Staphylococcus aureus pathogenesis
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DOI：
10.1128/iai.71.4.2208-2217.2003
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发表时间：
2003-04-01
影响因子：
3.1
通讯作者：
Calderwood, SB
中科院分区：
文献类型：
--
作者：
Sifri, CD;Begun, J;Calderwood, SB
金黄色葡萄球菌是人类和其他温血动物的重要病原体，也能够杀死线虫秀丽隐杆线虫。在这里，我们表明，C。elegans有机体，以S.金黄色葡萄球菌在与线虫消化道内细菌积累相关的过程中在数天内死亡。几个S。已知或推测在线虫致病中重要的金黄色葡萄球菌毒力决定因子，包括群体感应全局毒力调节系统agr和全局毒力调节因子sarA、替代性σ因子σ（B）、α-溶血素和V8丝氨酸蛋白酶，是线虫完全致病所必需的。此外，还介绍了几种已定义的C.检测了线虫突变体对S.金黄色葡萄球菌感染。对S.在C.线虫基因esp-2/sek-1和esp-8/nsy-1，其编码参与线虫防御多种病原体的保守p38 MAP激酶信号传导途径的组分。这些结果表明，S。金黄色葡萄球菌的致病机制是保守的，与宿主无关，并且特异性C.线虫宿主因素可以改变对这种革兰氏阳性人类病原体的易感性。
Staphylococcus aureus, an important pathogen of humans and other warm-blooded animals, is also capable of killing the nematode Caenorhabditis elegans. Here, we show that C. elegans organisms that are fed S. aureus die over the course of several days in a process that is correlated with the accumulation of bacteria within the nematode digestive tract. Several S. aureus virulence determinants known or speculated to be important in rnammalian pathogenesis, including the quorum-sensing global virulence regulatory system agr and the global virulence regulator sarA, the alternative sigma factor sigma(B), alpha-hemolysin, and V8 serine protease, are required for full pathogenicity in nematodes. In addition, several defined C. elegans mutants were examined for susceptibility to S. aureus infection. Enhanced susceptibility to S. aureus killing was observed with loss-of-function mutations in the C. elegans genes esp-2/sek-1 and esp-8/nsy-1, which encode components of a conserved p38 MAP kinase signaling pathway involved in nematode defense against multiple pathogens. These results suggest that key aspects of S. aureus pathogenesis have been conserved, irrespective of the host, and that specific C. elegans host factors can alter susceptibility to this gram-positive human pathogen.