Asiatic acid protects oocytes against in vitro aging-induced deterioration and improves subsequent embryonic development in pigs.
Asiatic acid protects oocytes against in vitro aging-induced deterioration and improves subsequent embryonic development in pigs.
复制标题
积雪草酸可保护卵母细胞免受体外衰老引起的退化，并改善猪随后的胚胎发育
DOI：
10.18632/aging.202184
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发表时间：
2020-12-03
期刊：
影响因子：
--
通讯作者：
Liang S
中科院分区：
文献类型：
--
作者：
Hu WY;Li XX;Diao YF;Qi JJ;Wang DL;Zhang JB;Sun BX;Liang S
积雪草酸(AA)是积雪草中的一种五环三萜类化合物，具有多种生物活性，是一种高效的抗氧化剂。在目前的研究中，我们调查了AA是否有潜力挽救在体外老化(IVA)过程中发生的猪卵母细胞质量下降。收集成熟猪卵母细胞，在成熟培养液中分别加入或不加入AA，继续培养24 h或48 h，作为IVA模型。结果表明，添加AA可降低IVA过程中猪卵母细胞的异常老化百分率。此外，添加AA有效地维持了老化猪卵母细胞的发育能力，包括孤雌激活和体外受精。添加AA组与老化猪卵母细胞透明带结合的精子数显著高于未添加组。此外，添加AA不仅能阻断IVA诱导的氧化应激，还能维持细胞内GSH水平，降低老化猪卵母细胞早期凋亡率。在IVA过程中，线粒体功能紊乱。添加AA可显著提高老化猪卵母细胞内ATP水平和线粒体膜电位。因此，AA对猪IVA过程中卵母细胞的质量和发育潜力的维持有一定的作用。
As a pentacyclic triterpene in Centella asiatica, asiatic acid (AA) is a powerful antioxidant with many bioactivities. In the present research, we investigated whether AA has the potential to rescue the decrease in porcine oocyte quality that occurs during in vitro aging (IVA). Mature porcine oocytes were collected and then continuously cultured for an additional 24 h or 48 h with or without AA in maturation medium as an IVA model. The results revealed that AA supplementation reduced the percentage of abnormal aged porcine oocytes during IVA. Furthermore, AA supplementation effectively maintained aged porcine oocyte developmental competence, both parthenogenetic activation and in vitro fertilization. The number of sperm that bound to the zona pellucida on aged porcine oocytes was higher in the AA-supplemented group than in the non-supplemented group. Moreover, AA supplementation not only blocked IVA-induced oxidative stress but also maintained intracellular GSH levels and reduced the percentage of early apoptosis aged porcine oocytes. Mitochondrial functions were disordered during the IVA process. The intracellular ATP levels and mitochondrial membrane potential in aged porcine oocytes were dramatically increased by AA supplementation. Therefore, AA has beneficial effects on porcine oocyte quality and developmental potential maintenance during IVA.