PhotothermalPhage: A Virus-Based Photothermal Therapeutic Agent
PhotothermalPhage: A Virus-Based Photothermal Therapeutic Agent
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光热噬菌体：一种基于病毒的光热治疗剂
DOI：
10.1021/jacs.1c05090
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发表时间：
2021
影响因子：
15
通讯作者：
Parsamian, Perouza
中科院分区：
文献类型：
--
作者：
Shahrivarkevishahi, Arezoo;Luzuriaga, Michael A.;Herbert, Fabian C.;Tumac, Alisia C.;Brohlin, Olivia R.;Wijesundara, Yalini H.;Adlooru, Abhinay V.;Benjamin, Candace;Lee, Hamilton;Parsamian, Perouza
病毒样颗粒（VLP）是模拟病毒结构的多功能纳米载体。它们可以作为特定功能化和免疫的安全平台，在广泛的生物医学应用中提供益处。在这项工作中，开发了新一代免疫光热剂，其使用称为噬菌体Qβ的化学修饰的VLP辅助光热消融。该设计是基于近红外吸收croconium染料与位于Qβ表面的赖氨酸残基的缀合，这将其转化为一种强大的近红外吸收剂，称为光热噬菌体。该系统可以在808 nm NIR激光辐射下产生比游离染料更多的热量，并且具有与金纳米结构相当的光热效率，但它是可生物降解的，并且与其产生的热量结合作为免疫佐剂。热消融与VLP的轻度免疫原性的协同组合导致原发性肿瘤的有效抑制、肺转移的减少和存活时间的增加。
Virus-like particles (VLPs) are multifunctional nanocarriers that mimic the architecture of viruses. They can serve as a safe platform for specific functionalization and immunization, which provides benefits in a wide range of biomedical applications. In this work, a new generation immunophotothermal agent is developed that adjuvants photothermal ablation using a chemically modified VLP called bacteriophage Qβ. The design is based on the conjugation of near-infrared absorbing croconium dyes to lysine residues located on the surface of Qβ, which turns it to a powerful NIR-absorber called PhotothermalPhage. This system can generate more heat upon 808 nm NIR laser radiation than free dye and possesses a photothermal efficiency comparable to gold nanostructures, yet it is biodegradable and acts as an immunoadjuvant combined with the heat it produces. The synergistic combination of thermal ablation with the mild immunogenicity of the VLP leads to effective suppression of primary tumors, reduced lung metastasis, and increased survival time.