Susceptibility of human T-lymphotropic virus type I infected cell line MT-2 to hepatitis C virus infection.
Susceptibility of human T-lymphotropic virus type I infected cell line MT-2 to hepatitis C virus infection.
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人类 T 淋巴细胞病毒 I 型感染细胞系 MT-2 对丙型肝炎病毒感染的敏感性。
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发表时间：
1995
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通讯作者：
K. Shimotohno
中科院分区：
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作者：
Nobuyuki Kato;Takahide Nakazawa;Tetsuya Mizutani;K. Shimotohno
为了获得丙型肝炎病毒（HCV）增殖系统，我们检查了各种培养细胞系对HCV感染的易感性。我们发现，人T淋巴细胞病毒I型感染的细胞系MT-2对HCV感染相当敏感。使用聚合酶链反应，细胞内阳性链HCV RNA检测，直到至少15天后接种（p.i.）。在感染后10天也检测到细胞内负链HCV RNA，虽然不是在感染后7天，表明HCV在感染后10天在MT-2细胞中复制。高变区1（HVR 1）的序列分析显示，来自感染后10天的细胞的HVR 1序列与来自感染后10天的细胞的HVR 1序列相似。已成为同质的，虽然HVR 1序列从接种物显示出典型的准种。我们还发现缺乏针对HVR 1种类的抗HVR 1抗体，其在感染后10天变得同质，尽管我们很容易检测到从接种物中获得的抗其它HVR 1种类的抗体。这些发现表明MT-2细胞对HCV感染敏感，并且能够支持HCV复制。
To obtain a hepatitis C virus (HCV) proliferation system, we examined the susceptibility of various cultured cell lines to HCV infection. We found that a human T-lymphotropic virus type I infected cell line MT-2 was fairly sensitive to HCV infection. Using the polymerase chain reaction, intracellular positive-stranded HCV RNA was detected until at least 15 days postinoculation (p.i.). Intracellular negative-stranded HCV RNA was also detected at 10 days p.i., although not at 7 days p.i., suggesting that HCV is replicating in MT-2 cells 10 days p.i. Sequence analysis of hypervariable region 1 (HVR1) revealed that HVR1 sequences from cells 10 days p.i. had become homogeneous, although HVR1 sequences from the inoculum showed the typical quasi-species. We also found a lack of anti-HVR1 antibody against the HVR1 species which became homogeneous at 10 days p.i., although we easily detected antibody against the other HVR1 species obtained from the inoculum. These findings suggest that MT-2 cells are susceptible to HCV infection and are capable of supporting HCV replication.