Decreased lung carcinoma cell functions on select polymer nanometer surface features.
Decreased lung carcinoma cell functions on select polymer nanometer surface features.
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DOI：
10.1002/jbm.a.33217
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Lijuan Zhang;T. Webster
中科院分区：
文献类型：
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作者：
Lijuan Zhang;T. Webster
生物材料纳米形貌已经被提出作为显著影响细胞功能（包括成骨细胞、成纤维细胞、内皮细胞、软骨细胞、免疫细胞和细菌）的手段。在这项研究中，肺上皮癌细胞的功能，包括粘附（最多4小时），增殖（最多3天），凋亡（最多5天），血管内皮生长因子（VEGF）的合成（最多5天）的聚乳酸-共-乙醇酸（PLGA）薄膜与各种纳米形貌进行了系统的研究。重要的是，这项研究创建了具有各种纳米形貌（特别是纳米光滑，23，300和400 nm半球形表面特征）但表面化学相似的PLGA膜，以便仅关注PLGA形貌对癌细胞功能的影响。简单有效的铸造成型和溶剂蒸发方法被用来实现这一点。原子力显微镜，电子光谱化学分析，和水接触角测量验证了类似的表面化学和能量，但不同的拓扑结构在这项研究中制备的所有PLGA薄膜。肺上皮癌细胞粘附、增殖和形态学结果表明，与任何其他样品相比，纳米光滑和400 nm表面特征的PLGA上的细胞生长和扩散较少。然而，结果还表明，与纳米光滑基底相比，23 nm表面特征PLGA上的肺上皮癌细胞VEGF（分泌用于肿瘤血管形成的关键生长因子）合成减少长达5天。总之，这些结果提供了第一个见解，了解PLGA nanotography在介导肺癌细胞功能中可能发挥的作用，广泛应用于再生医学。
Biomaterial nanotopographies have been proposed as a means to significantly influence cell functions (including osteoblasts, fibroblasts, endothelial cells, chondrocytes, immune cells, and bacteria). In this study, lung epithelial carcinoma cell functions including adhesion (up to 4 h), proliferation (up to 3 days), apoptosis (up to 5 days), and vascular endothelial growth factor (VEGF) synthesis (up to 5 days) on poly-lactic-co-glycolic (PLGA) films with various nanotopographies were systematically investigated. Importantly, this study created PLGA films with various nanotopographies (specifically, nano-smooth, 23, 300, and 400 nm hemispherical surface features) but similar surface chemistry in order to focus only on the effect of PLGA topography on cancer cell functions. Simple and effective cast-molding and solvent evaporation methods were used to accomplish this. Atomic force microscopy, electron spectroscopy for chemical analysis, and water contact angle measurements verified similar surface chemistry and energy but varied topographies for all of the PLGA films prepared in this study. Lung epithelial carcinoma cell adhesion, proliferation, and morphology results indicated less cell growth and spreading on nano-smooth and 400 nm surface-featured PLGA compared to any other samples. However, results also demonstrated decreased lung epithelial carcinoma cell VEGF (a key growth factor secreted for the vascularization of tumors) synthesis on the 23 nm surface-featured PLGA compared to the nano-smooth substrates for up to 5 days. In summary, these results provided the first insights into understanding the role that PLGA nanotography may play in mediating lung carcinoma cell functions for a wide range of applications in regenerative medicine.