THIK-1 (K2P13.1) is a small-conductance background K(+) channel in rat trigeminal ganglion neurons.
THIK-1 (K2P13.1) is a small-conductance background K(+) channel in rat trigeminal ganglion neurons.
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DOI：
10.1007/s00424-013-1358-1
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发表时间：
2014-07
影响因子：
4.5
通讯作者：
Kim, Donghee
中科院分区：
文献类型：
--
作者：
Kang, Dawon;Hogan, James O.;Kim, Donghee
本研究的目的是确定在三叉神经节神经元中表达的小电导（~5-pS）背景K+通道的分子身份。我们通过比较HEK 293细胞中表达的THIK-1的药理学和单通道特性来检验5-pS通道是K2 P通道的假设。如前所述，全细胞THIK-1电流被氟烷抑制，被花生四烯酸激活。在测试的25种另外的调节剂中，布比卡因（100 μM）、奎尼丁（50 μM）和Ba 2+（3 mM）以及冷（10 ℃）是THIK-1电流的最有效抑制剂（>50%抑制）。在移液器和浴槽溶液中含有高KCl的细胞贴附贴片中，THIK-1产生了一个小电导（~5 pS）通道，具有弱的内向整流电流-电压关系。氟烷，布比卡因和冷抑制TG神经元THIK-1和5-pS通道的单通道活动，而花生四烯酸增强它们。HEK 293细胞表达THIK-1，TG神经元5-pS通道对缺氧不敏感。RT-PCR、Western blot和免疫细胞化学分析结果表明，TG神经元表达THIK-1 mRNA和蛋白。这些结果表明THIK-1在TG神经元中功能性表达，并有助于背景K+电导。
The goal of this study was to determine the molecular identity of a small conductance (~5-pS) background K+ channel expressed in trigeminal ganglion neurons. We tested the hypothesis that the 5-pS channel is a K2P channel by comparing the pharmacological and single-channel properties of THIK-1 expressed in HEK293 cells. As reported earlier, whole-cell THIK-1 current was inhibited by halothane and activated by arachidonic acid. Among 25 additional modulators tested, bupivacaine (100 μM), quinidine (50 μM) and Ba2+ (3 mM) and cold (10°C) were most effective inhibitors of THIK-1 current (>50% inhibition). In cell-attached patches with high KCl in the pipette and bath solutions, THIK-1 produced a small conductance (~5-pS) channel with a weak inwardly rectifying current-voltage relationship. Halothane, bupivacaine and cold inhibited the single-channel activities of both THIK-1 and the 5-pS channel in TG neurons, whereas arachidonic acid augmented them. THIK-1 expressed in HEK293 cells and the 5-pS channels in TG neurons were insensitive to hypoxia. Reverse transcriptase-PCR, western blot and immunocytochemical analyses suggested that THIK-1 mRNA and protein were expressed in TG neurons. These results show that THIK-1 is functionally expressed in TG neurons and contributes to the background K+ conductance.
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DOI：
10.1523/jneurosci.1956-10.2010
发表时间：
2010-07-07
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Lazarenko RM;Fortuna MG;Shi Y;Mulkey DK;Takakura AC;Moreira TS;Guyenet PG;Bayliss DA
通讯作者：
Bayliss DA
影响因子：
5.5
作者：
Kang, DW;Han, JH;Kim, DH
通讯作者：
Kim, DH
影响因子：
5.3
作者：
Kim, Donghee;Cavanaugh, Eric J.
通讯作者：
Cavanaugh, Eric J.
影响因子：
5.5
作者：
Kang, Dawon;Kim, Donghee
通讯作者：
Kim, Donghee
DOI：
10.1152/ajprenal.00132.2006
发表时间：
2007-02-01
影响因子：
4.2
作者：
L'Hoste, S.;Barriere, H.;Poujeol, P.
通讯作者：
Poujeol, P.