Reggie-1 and reggie-2, two cell surface proteins expressed by retinal ganglion cells during axon regeneration.
Reggie-1 and reggie-2, two cell surface proteins expressed by retinal ganglion cells during axon regeneration.
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发表时间：
1997-01
期刊：
影响因子：
4.6
通讯作者：
T. Schulte;Katja A. Paschke;U. Laessing;F. Lottspeich;C. Stuermer
中科院分区：
文献类型：
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作者：
T. Schulte;Katja A. Paschke;U. Laessing;F. Lottspeich;C. Stuermer
与哺乳动物相反，鱼类在视神经被切断时再生视网膜神经节细胞轴突。视神经损伤导致蛋白质的再表达，其通常首先在新分化的视网膜神经节细胞和轴突中表达。本研究利用单克隆抗体M802和分子克隆技术，从鱼类视网膜神经节细胞中鉴定出两个新蛋白，reggie-1和reggie-2。在正常鱼类中，M802染色了来自新生神经节细胞的少数视网膜轴突，这些神经节细胞在鱼类中终身添加到视网膜边缘。然而，在视神经损伤后，M802标记了所有视网膜神经节细胞和视网膜轴突进入顶盖的整个路径。与M802染色一致，原位杂交证明reggie-1和reggie-2 mRNA存在于受损的视网膜神经节细胞中，但在正常成熟的对应细胞中检测不到。在用成年金鱼脑的膜蛋白进行的蛋白质印迹中，M802识别48 x10（3）Mr的蛋白质条带。在氨基酸水平上，48 × 10（3）Mr reggie-1和reggie-2有44%相同，缺乏跨膜和膜锚结构域，但似乎通过离子相互作用与膜结合。Reggie-1和reggie-2与35 x10（3）Mr ESA（人表皮表面抗原）同源，但在此被鉴定为神经元表面蛋白，存在于视网膜边缘的新分化的神经节细胞上，并且在损伤后和轴突再生期间在成熟的神经节细胞中重新表达。
Fish--in contrast to mammals--regenerate retinal ganglion cell axons when the optic nerve is severed. Optic nerve injury leads to reexpression of proteins, which typically are first expressed in newly differentiated retinal ganglion cells and axons. Here we identified two new proteins of fish retinal ganglion cells, reggie-1 and reggie-2, with monoclonal antibody M802 and molecular cloning techniques. In normal fish, M802 stained the few retinal axons derived from newborn ganglion cells which in fish are added lifelong to the retinal margin. After optic nerve injury, however, M802 labeled all retinal ganglion cells and retinal axons throughout their path into tectum. Consistent with M802 staining, reggie-1 and reggie-2 mRNAs were present in lesioned retinal ganglion cells, as demonstrated by in situ hybridization, but were not detectable in their normal mature counterparts. In western blots with membrane proteins of the adult goldfish brain, M802 recognizes a 48x10(3) Mr protein band. At the amino acid level, 48x10(3) Mr reggie-1 and reggie-2 are 44% identical, lack transmembrane and membrane anchor domains, but appear membrane associated by ionic interactions. Reggie-1 and reggie-2 are homologous to 35x10(3) Mr ESA (human epidermal surface antigen) but are here identified as neuronal surface proteins, present on newly differentiated ganglion cells at the retinal margin and which are reexpressed in mature ganglion cells upon injury and during axonal regeneration.