Time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy for nondestructive evaluation of vascular grafts
Time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy for nondestructive evaluation of vascular grafts
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DOI：
10.1117/1.jbo.19.8.080503
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发表时间：
2014-08-01
影响因子：
3.5
通讯作者：
Marcu, Laura
中科院分区：
文献类型：
--
作者：
Fatakdawala, Hussain;Griffiths, Leigh G.;Marcu, Laura
结构和组成的定量和定性评价在体外和体内监测工程化血管组织的发育中是重要的。破坏性技术是对单个样品或动物进行延时分析的障碍。这项研究表明，时间分辨荧光光谱（TRFS）和超声背散射显微镜（UBM），作为无损和协同技术，组织移植物的成分和形态分析的能力，分别。UBM图像和背向散射积分系数证明了可视化和量化血管移植物生物材料植入后变化的能力，例如移植区域的外弹性膜丢失和内膜/中膜增厚以及移植物与周围组织的整合。TRFS结果表明，由于正常和移植组织区域之间的胶原蛋白，弹性蛋白和细胞含量的变化，光谱，平均寿命和荧光衰减参数的显着变化。这些结果奠定了应用基于导管的技术在体内评价血管移植物TRFS和UBM的基础。(C)2014年，美国光电仪器工程师学会（SPIE）
Quantitative and qualitative evaluations of structure and composition are important in monitoring development of engineered vascular tissue both in vitro and in vivo. Destructive techniques are an obstacle for performing time-lapse analyses from a single sample or animal. This study demonstrates the ability of time-resolved fluorescence spectroscopy (TRFS) and ultrasound back-scatter microscopy (UBM), as nondestructive and synergistic techniques, for compositional and morphological analyses of tissue grafts, respectively. UBM images and integrated backscatter coefficients demonstrate the ability to visualize and quantify postimplantation changes in vascular graft biomaterials such as loss of the external elastic lamina and intimal/medial thickening over the grafted region as well as graft integration with the surrounding tissue. TRFS results show significant changes in spectra, average lifetime, and fluorescence decay parameters owing to changes in collagen, elastin, and cellular content between normal and grafted tissue regions. These results lay the foundation for the application of a catheter-based technique for in vivo evaluation of vascular grafts using TRFS and UBM. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)