Highly flexible heparin-modified chitosan/graphene oxide hybrid hydrogel as a super bilirubin adsorbent with excellent hemocompatibility.
Highly flexible heparin-modified chitosan/graphene oxide hybrid hydrogel as a super bilirubin adsorbent with excellent hemocompatibility.
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DOI：
10.1039/c4tb01673d
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发表时间：
2015-02
期刊：
影响因子：
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通讯作者：
Houliang Wei;Lulu Han;Yongchao Tang;Jun Ren;Zongbin Zhao;Lingyun Jia
中科院分区：
文献类型：
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作者：
Houliang Wei;Lulu Han;Yongchao Tang;Jun Ren;Zongbin Zhao;Lingyun Jia
胆红素作为一种致病毒素，通常通过血液灌流从血液中去除，以缓解肝病或为等待肝移植的患者争取时间。然而，开发具有优良机械性能、吸附性能和血液相容性的胆红素吸附剂仍是一个相当大的挑战。在这项工作中，肝素修饰的壳聚糖/氧化石墨烯杂化水凝胶（hep-CS/GH）已开发用于胆红素吸附使用冻干-中和-修饰策略。所制备的杂化水凝胶显示出独特的泡沫状多孔结构和优异的机械柔性。结果表明，将氧化石墨烯掺入到壳聚糖基质中增强了混合水凝胶的抗压强度和杨氏模量，以及其对胆红素的吸附能力。hep-CS/GH对胆红素的最大吸附容量为92.59 mg g-1，符合Langmuir吸附等温式。结果表明，hep-CS/GH成功地与白蛋白竞争，并能有效地从富含胆红素的血清中吸附胆红素。经肝素修饰后，hep-CS/GH的蛋白质吸附、血小板粘附和溶血作用均降低，血浆凝固时间由4.1 min延长至23.6 min，表明hep-CS/GH具有良好的上级血液相容性。因此，本研究可为提高吸附剂去除血液毒素的性能铺平道路。
As a pathogenic toxin, bilirubin is generally removed from blood by hemoperfusion for the remission of liver disease or to gain time for patients waiting for liver transplantation. However, the development of bilirubin adsorbents with excellent mechanical properties, adsorption performance and hemocompatibility is still a considerable challenge. In this work, a heparin-modified chitosan/graphene oxide hybrid hydrogel (hep-CS/GH) has been developed for bilirubin adsorption using a lyophilization-neutralization-modification strategy. The as-prepared hybrid hydrogel displayed a unique foam-like porous structure and excellent mechanical flexibility. It was revealed that the incorporation of graphene oxide into the chitosan matrix enhanced both the compressive strength and the Young's modulus of the hybrid hydrogel, as well as its adsorption capacity for bilirubin. The maximum adsorption capacity of hep-CS/GH for bilirubin was 92.59 mg g-1, according to the Langmuir isotherm model. It was demonstrated that hep-CS/GH successfully competed with albumin, and could effectively adsorb bilirubin from a bilirubin-enriched serum. After the hydrogel was modified with heparin, protein adsorption, platelet adhesion and hemolysis were reduced, and the plasma clotting time was prolonged from 4.1 to 23.6 min, indicating the superior hemocompatibility of hep-CS/GH. Therefore, this study may pave the way for improving the performance of the adsorbent in removing blood toxins.