Preparation of 2‐amino‐2‐hydroxymethyl‐1,3‐propanediol ( TRIS ) pHT buffers in synthetic seawater
Preparation of 2‐amino‐2‐hydroxymethyl‐1,3‐propanediol ( TRIS ) pHT buffers in synthetic seawater
复制标题
合成海水中 2-氨基-2-羟甲基-1,3-丙二醇 (TRIS) pHT 缓冲液的制备
DOI：
10.1002/lom3.10383
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Dickson, Andrew G.
中科院分区：
文献类型：
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作者：
Paulsen, May‐Linn;Dickson, Andrew G.
用于校准海水 pHT 测量的已知质量的缓冲液尚未广泛或市售。尽管已经发表了合成海水中 0.04 mol kg-H2O−1 等摩尔缓冲液 2-氨基-2-羟甲基-1,3-丙二醇 (TRIS)-TRIS · H+ 的组合物，但没有明确的程序描述制备该缓冲液以达到具有已知不确定性的特定 pHT。这里描述了这样的程序，它利用容易获得的实验室设备和技术来生产缓冲液，其pHT在DelValls和Dickson最初指定的已发表pHT值（1998）的0.006以内，在25°C时为8.094。这样的缓冲液将适合校准 pH 测量值，以实现全球海洋酸化观测网络 pHT 为 0.02 的“天气”不确定性目标，这一不确定性目标适合“识别相对空间模式和短期变化”。
Buffers of known quality for the calibration of seawater pHTmeasurements are not widely or commercially available. Although there exist published compositions for the 0.04 mol kg‐H2O−1equimolar buffer 2‐amino‐2‐hydroxymethyl‐1,3‐propanediol (TRIS)‐TRIS · H+in synthetic seawater, there are no explicit procedures that describe preparing this buffer to achieve a particular pHTwith a known uncertainty. Such a procedure is described here which makes use of easily acquired laboratory equipment and techniques to produce a buffer with a pHTwithin 0.006 of the published pHTvalue originally assigned by DelValls and Dickson (1998), 8.094 at 25°C. Such a buffer will be suitable for the calibration of pH measurements expected to fulfil the “weather” uncertainty goal of the Global Ocean Acidification Observation Network of 0.02 in pHT, an uncertainty goal appropriate to “identify relative spatial patterns and short‐term variation.”