Y-shaped RNA Secondary Structure of a Noncoding Region in the Genomic RNA of Tick-Borne Encephalitis Virus Affects Pathogenicity.
Y-shaped RNA Secondary Structure of a Noncoding Region in the Genomic RNA of Tick-Borne Encephalitis Virus Affects Pathogenicity.
复制标题
蜱传脑炎病毒基因组RNA非编码区的Y形RNA二级结构影响致病性。
DOI：
10.1111/1348-0421.12971
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发表时间：
2022
影响因子：
2.6
通讯作者：
Yoshii K
中科院分区：
文献类型：
--
作者：
Nishiyama S;Hirano M;Muto M;Kambara M;Ito N;Kobayashi S;Kariwa H;Yoshii K
蜱传脑炎病毒（TBEV）是一种引起人类脑炎的人畜共患病毒。在TBEV基因组3′非翻译区的可变区已经报道了多种缺失。本研究通过反向遗传学分析了Y形二级结构在TBEV致病性中的作用。该结构的缺失增加了病毒感染小鼠的死亡率，但不影响培养细胞和器官中的病毒增殖。结果表明，二级结构参与了TBEV致病的调控。
Tick‐borne encephalitis virus (TBEV) is a zoonotic virus that causes encephalitis in humans. Various deletions have been reported in a variable region of the 3′ untranslated region of the TBEV genome. This study analyzed the role of a Y‐shaped secondary structure in the pathogenicity of TBEV by using reverse genetics. Deletion of the structure increased the mortality rate of virus‐infected mice but did not affect the virus multiplication in cultured cells and organs. The results indicate that the secondary structure is involved in the regulation of TBEV pathogenesis.