A serum protein adsorption profile on BCP ceramics and influence of the elevated adsorption of adhesive proteins on the behaviour of MSCs.
A serum protein adsorption profile on BCP ceramics and influence of the elevated adsorption of adhesive proteins on the behaviour of MSCs.
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DOI：
10.1039/c8tb02283f
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发表时间：
2018-11
期刊：
影响因子：
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通讯作者：
Jing Wang;Xuening Chen;Bo Guo;Xiao Yang;Yong Zhou;Xiangdong Zhu;Kai Zhang;Yujiang Fan;
中科院分区：
文献类型：
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作者：
Jing Wang;Xuening Chen;Bo Guo;Xiao Yang;Yong Zhou;Xiangdong Zhu;Kai Zhang;Yujiang Fan;
蛋白质吸附通过影响细胞行为在骨修复和再生中起关键作用。本研究选择具有优异成骨性的双相磷酸钙（BCP）陶瓷，采用等压标签相对和绝对定量（iTRAQ）蛋白质组学技术研究其血清蛋白吸附谱。结合基因本体（GO）注释和京都基因与基因组百科全书（KEGG）分析，对281个差异吸附的血清蛋白及其参与的生物过程进行了确认。进一步选择细胞外基质(ECM)-受体相互作用通路中差异吸附的粘附蛋白，研究其在间充质干细胞（MSCs）行为中的作用。预包衣和阻断实验表明，吸附的玻璃体连接蛋白（VN）和层粘连蛋白（LN）均可通过与不同整合素亚基的相互作用促进MSCs在BCP陶瓷上的附着、增殖和成骨分化。结果表明，整合素α2、αv和β3、β5的表达上调可能参与了vn介导的MSC功能，α6和β1、β4的基因表达上调可能与ln参与的MSC功能相关。上述结果证明，在材料介导的骨诱导过程中，蛋白质在生物材料上的优先吸附对调节间充质干细胞功能至关重要。
Protein adsorption plays a key role in bone repair and regeneration by affecting cell behavior. In this study, a biphasic calcium phosphate (BCP) ceramic, with excellent osteoinductivity, was chosen to investigate its serum protein adsorption profile using isobaric tags for relative and absolute quantification (iTRAQ) proteomics technology. 281 differentially adsorbed serum proteins and the involved biological processes were confirmed by the combination of Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The differentially adsorbed adhesive proteins in the extracellular matrix (ECM)-receptor interaction pathway were further selected to investigate their roles in the behavior of mesenchymal stem cells (MSCs). Pre-coating and blockage experiments revealed that both adsorbed vitronectin (VN) and laminin (LN) could promote the attachment, proliferation and osteogenic differentiation of MSCs on the BCP ceramic by interacting with different integrin subunits. It is revealed that the up-regulated expressions of integrin α2, αv and β3, β5 could contribute to VN-mediated MSC functions, and the elevated gene expressions of α6 and β1, β4 could be related to the LN-participated process. The above results proved that the preferential protein adsorption on a biomaterial should be vital for modulating MSC functions in the course of material-mediated osteoinductivity.