Protein changes during programmed cell death in tobacco
Protein changes during programmed cell death in tobacco
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DOI：
10.1007/s10535-011-0021-y
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发表时间：
2011-03-01
影响因子：
1.5
通讯作者：
Regalado, A. P.
中科院分区：
文献类型：
--
作者：
Chaves, I.;Alves, M.;Regalado, A. P.
用Yariv试剂诱导烟草（Nicotiana tabacum cv.亮黄色-2细胞悬液。蛋白质提取物的2-D电泳分析清楚地表明，大量的蛋白质降解，这主要是由于半胱氨酸蛋白酶活性。相反，一些蛋白质在PCD诱导后72 h保持不变。这些蛋白质的肽质量指纹图谱，通过MALDI-TOF，确定钙网蛋白，热休克蛋白（HSP）60，HSP 70，苹果酸脱氢酶和线粒体ATP合成酶β亚基。
Programmed cell death (PCD) was induced by the Yariv reagent in Nicotiana tabacum cv. Bright Yellow-2 cell suspension. The analyses of proteins extracts by 2-D electrophoresis clearly show massive protein degradation which was mainly due to cysteine protease activity. In contrast, some proteins remained unchanged up to 72 h after PCD induction. Peptide mass fingerprints of these proteins, obtained by MALDI-TOF, identified calreticulin, heat shock protein (HSP) 60, HSP70, malate dehydrogenase and mitochondrial ATP synthase beta-subunit.