Identification of stress-tolerance-related transcription-factor genes via mini-scale Full-length cDNA Over-eXpressor (FOX) gene hunting system
Identification of stress-tolerance-related transcription-factor genes via mini-scale Full-length cDNA Over-eXpressor (FOX) gene hunting system
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DOI：
10.1016/j.bbrc.2007.09.124
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发表时间：
2007-12-14
影响因子：
3.1
通讯作者：
Shinozaki, Kazuo
中科院分区：
文献类型：
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作者：
Fujita, Miki;Mizukado, Saho;Shinozaki, Kazuo
最近，我们开发了一种称为全长 cDNA Over-eXpressor (FOX) 基因狩猎的新型系统 [T. Ichikawa、M. Nakazawa、M. Kawashima、H. Iizumi、H. Kuroda、Y. Kondou、Y. Tsuhara、K. Suzuki、A. Ishikawa、M. Seki、M. Fujita、R. Motohashi、N. Nagata、T. Takagi、K. Shinozaki、M. Matsui，FOX 狩猎系统：另一种功能获得性基因狩猎技术，Plant J. 48 (2006) 974-985]，涉及标准化拟南芥全长 cDNA 文库的随机过表达。虽然我们的系统允许大规模收集全长 cDNA 以进行基因发现，但我们试图缩小其规模以分析一小部分全长 cDNA。作为模型系统，我们关注压力诱导的转录因子。将 43 个胁迫诱导转录因子的全长 cDNA 混合以创建转基因植物文库。我们在T1代中筛选了耐盐胁迫品系，并鉴定了许多含有相同转基因（F39）的耐盐品系。 F39 编码与 AtbZIP60 相同的 bZIP 型转录因子，据信 AtbZIP60 参与内质网应激反应。微阵列分析显示，F39 过表达株系中许多应激诱导基因上调，表明 AtbZIP60 参与应激信号转导。因此，我们的小型 FOX 系统可用于从一小部分表现出相似表达谱的基因中筛选具有有价值功能的基因，例如转录因子。 (C) 2007 Elsevier Inc. 保留所有权利。
Recently, we developed a novel system known as Full-length cDNA Over-eXpressor (FOX) gene hunting [T. Ichikawa, M. Nakazawa, M. Kawashima, H. Iizumi, H. Kuroda, Y. Kondou, Y. Tsuhara, K. Suzuki, A. Ishikawa, M. Seki, M. Fujita, R. Motohashi, N. Nagata, T. Takagi, K. Shinozaki, M. Matsui, The FOX hunting system: an alternative gain-of-function gene hunting technique, Plant J. 48 (2006) 974-985], which involves the random overexpression of a normalized Arabidopsis full-length cDNA library. While our system allows large-scale collection of full-length cDNAs for gene discovery, we sought to downsize it to analyze a small pool of full-length cDNAs. As a model system, we focused on stress-inducible transcription factors. The full-length cDNAs of 43 stress-inducible transcription factors were mixed to create a transgenic plant library. We screened for salt-stress-resistant lines in the T1 generation and identified a number of salt-tolerant lines that harbored the same transgene (F39). F39 encodes a bZIP-type transcription factor that is identical to AtbZIP60, which is believed to be involved in the endoplasmic reticulum stress response. Microarray analysis revealed that a number of stress-inducible genes were up-regulated in the F39-overexpressing lines, suggesting that AtbZIP60 is involved in stress signal transduction. Thus, our mini-scale FOX system may be used to screen for genes with valuable functions, such as transcription factors, from a small pool of genes that show similar expression profiles. (C) 2007 Elsevier Inc. All rights reserved.