Differential effect of halide anions on the hydrolysis of different dansyl substrates by thermolysin.
Differential effect of halide anions on the hydrolysis of different dansyl substrates by thermolysin.
复制标题
卤化物阴离子对嗜热菌蛋白酶水解不同丹磺酰底物的不同影响。
DOI：
10.1021/bi00187a019
复制
发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
VanWart,HE
中科院分区：
文献类型：
--
作者：
Yang,JJ;Artis,DR;VanWart,HE
MATERIALS AND METHODSMaterials. NaF, NaCl, NaBr, and Nal were purchased as spectrographically puresalts from Sigma. All other materials1 Abbreviations: Hepes, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; Tris, tris (hydroxymethyl) aminomethane; Mes, 2-(JV-morpholino)-ethanesulfonic acid; Dns or dansyl, 5-(dimethylamino)-naphthalenyl-lsulfonyl; Mns or mansyl, 6-(N-methylanilino)-2-naphthalenyl-1-sulfonyl; DE, direct excitation; ET, energy transfer; E, enzyme; S, substrate; P, product; ES, enzyme-substrate intermediate; FA, 2-furanacrylic acid; Bz, benzoyl; Cbz, benzyloxycarbony 1.
