Digital Holographic Microscopy, a new Method for Simultaneous Amplitude- and Quantitative Phase-Contrast Imaging
Digital Holographic Microscopy, a new Method for Simultaneous Amplitude- and Quantitative Phase-Contrast Imaging
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数字全息显微镜，一种同时进行幅度和定量相差成像的新方法
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
C. Depeursinge
中科院分区：
文献类型：
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作者：
E. Cuche;P. Marquet;P. Dahlgren;C. Depeursinge
数字全息显微术（DHM）是一种新的光学成像和分析方法，它涉及一种用于再现全息图的数值方法。离轴全息图的标本创建使用干涉设置和记录的CCD相机。数值重建的框架内进行的菲涅耳近似。我们在这里表明，DHM方法可以应用于同时重建的强度和相位分布的光场在微观样品的表面，具有相同的横向分辨率与经典的光学显微镜。我们还表明，一个重要的噪声源来自于使用高相干性的光源。
Digital Holographic Microscopy (DHM) is a new method for optical imaging and analysis that involves a numerical method for the reconstruction of holograms. An off-axis hologram of the specimen is created using an interferometric set-up and recorded by a CCD camera. The numerical reconstruction is performed within the framework of the Fresnel approximation. We show here that the DHM method can be applied to reconstruct simultaneously the distributions of intensity and phase of the optical field at the surface of microscopic specimen, with the same transverse resolution as with classical optical microscopy. We show also that an important source of noise comes from the use of a light source of high coherence.