The interaction of avian spermatozoa with the perivitelline layer in vitro and in vivo.
The interaction of avian spermatozoa with the perivitelline layer in vitro and in vivo.
复制标题
体外和体内禽类精子与卵周层的相互作用。
DOI：
10.1530/jrf.0.1010599
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
G. J. Wishart
中科院分区：
文献类型：
--
作者：
M. Steele;W. Meldrum;J. Brillard;G. J. Wishart
研究了鸡精子对卵周层内层的原位水解性和分离后的水解性。用荧光素标记的麦芽凝集素染色后，鸡精子在产蛋和新鲜排卵的卵黄周内层产生的水解点被视为暗孔。在活体受精的产卵中，这些洞更多地出现在动物极囊胚盘上的卵黄周层上，而不是植物极上。然而，在一项将新鲜排卵的卵黄周内层碎片与精子体外孵育的实验中，水解点出现在卵周层上，频率类似于动物极生发圆盘上方或植物极生发盘上方。从动物和植物极点的卵黄周内层提取的多肽的电泳谱没有差异。负责增加精子对动物极卵周层的吸引力或增加其活性的因素(S)似乎与卵黄周层本身无关。
The differences in the hydrolytic activity of chicken spermatozoa towards the inner perivitelline layer in situ and when isolated from the ovum were investigated. Points of hydrolysis produced by chicken spermatozoa on the inner perivitelline layer of laid and freshly ovulated eggs were viewed as dark holes after staining with fluorescein-conjugated wheat-germ agglutinin. In laid eggs that had been fertilized in vivo, these holes appeared more frequently on the perivitelline layer overlying the blastodisc at the animal pole than on that overlying the vegetal pole. However, in an assay in which fragments of the inner perivitelline layer from freshly ovulated eggs were incubated with spermatozoa in vitro, points of hydrolysis appeared with similar frequency on the perivitelline layer from over the germinal disc at the animal pole or from that over the vegetal pole. No difference could be demonstrated in the electrophoretic profile of peptides extracted from the inner perivitelline layer at the animal or vegetal pole. The factor(s) responsible for either increased attraction of spermatozoa to, or for increased activity at, the perivitelline layer of the animal pole does not appear to be associated with the perivitelline layer itself.