DNA Barcoding and Pharmacovigilance of Herbal Medicines
DNA Barcoding and Pharmacovigilance of Herbal Medicines
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DOI：
10.1007/s40264-015-0306-8
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发表时间：
2015-07-01
期刊：
影响因子：
4.2
通讯作者：
Newmaster, Steven G.
中科院分区：
文献类型：
--
作者：
de Boer, Hugo J.;Ichim, Mihael C.;Newmaster, Steven G.
草药的药物警戒依赖于有关成分的产品标签信息以及在商业化链上对良好生产规范的遵守。一些研究表明，草药中存在植物物种的替代，这反过来对草药药物警戒提出了挑战，因为不良反应可能是由于掺假或添加的成分造成的。使用分析化学方法对草药成分进行认证可以帮助检测污染物和毒素，但通常是有限的或无法检测污染源。利用DNA序列数据进行植物分子鉴定的最新进展，使得利用已定义的DNA标记从草药中准确鉴定植物物种成为可能。使用扩增子元条形码从复方中草药中鉴定多种成分物种，可以验证标记的成分和检测替代、掺假和添加的物种。DNA条形码已被证明是一种评估草药物种组成的有力方法，并有可能作为草药药物警戒研究中特定产品不良反应的标准方法。
Pharmacovigilance of herbal medicines relies on the product label information regarding the ingredients and the adherence to good manufacturing practices along the commercialisation chain. Several studies have shown that substitution of plant species occurs in herbal medicines, and this in turn poses a challenge to herbal pharmacovigilance as adverse reactions might be due to adulterated or added ingredients. Authentication of constituents in herbal medicines using analytical chemistry methods can help detect contaminants and toxins, but are often limited or incapable of detecting the source of the contamination. Recent developments in molecular plant identification using DNA sequence data enable accurate identification of plant species from herbal medicines using defined DNA markers. Identification of multiple constituent species from compound herbal medicines using amplicon metabarcoding enables verification of labelled ingredients and detection of substituted, adulterated and added species. DNA barcoding is proving to be a powerful method to assess species composition in herbal medicines and has the potential to be used as a standard method in herbal pharmacovigilance research of adverse reactions to specific products.