A sensitive DNA enzyme-based fluorescent assay for bacterial detection.
A sensitive DNA enzyme-based fluorescent assay for bacterial detection.
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DOI：
10.3390/biom3030563
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发表时间：
2013-08-20
期刊：
影响因子：
5.5
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Aguirre SD;Ali MM;Salena BJ;Li Y
细菌检测在保护公众健康和安全方面起着重要作用，因此，大量的研究工作已经针对开发敏感、特异、廉价和易于使用的细菌传感方法。我们最近报道了一种新的“混合-阅读”试验，其中荧光DNAzyme探针用于检测模式细菌E。杆菌在这项工作中，我们进行了一系列的优化实验，以提高该检测的性能。优化的测定可以实现1000个菌落形成单位（CFU）的检测限，而无需培养步骤，并且在生长培养基中细菌培养短至4小时后能够检测到1个CFU。总的来说，我们的努力已经导致了一个高度敏感和易于使用的荧光细菌检测试验，采用催化DNA的发展。
Bacterial detection plays an important role in protecting public health and safety, and thus, substantial research efforts have been directed at developing bacterial sensing methods that are sensitive, specific, inexpensive, and easy to use. We have recently reported a novel “mix-and-read” assay where a fluorogenic DNAzyme probe was used to detect model bacterium E. coli. In this work, we carried out a series of optimization experiments in order to improve the performance of this assay. The optimized assay can achieve a detection limit of 1000 colony-forming units (CFU) without a culturing step and is able to detect 1 CFU following as short as 4 h of bacterial culturing in a growth medium. Overall, our effort has led to the development of a highly sensitive and easy-to-use fluorescent bacterial detection assay that employs a catalytic DNA.