Exogenous Glucose Promotes Growth and Pectinase Activity of Bacillus licheniformis DY2 Through Frustrating the TCA Cycle
Exogenous Glucose Promotes Growth and Pectinase Activity of Bacillus licheniformis DY2 Through Frustrating the TCA Cycle
复制标题
外源葡萄糖通过抑制 TCA 循环促进地衣芽孢杆菌 DY2 的生长和果胶酶活性
DOI：
10.1007/s12257-019-0245-9
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发表时间：
2019-11
影响因子：
3.2
通讯作者：
Ye Xiuyun
中科院分区：
文献类型：
--
作者：
Du Xi;Wang Donghuang;Yin Di;Guan Yi;Ye Xiuyun
Microbial pectinases are important sources due to the ease of production and unique physicochemical properties. Here, DY2, a strain of Bacillus licheniformis, was identified from 14 strains of bacteria as a pectinase-producing bacterium with good application potential. Optimized carbon sources of submerged fermentation led to the identification of glucose as an ideal carbon source for activity and production of P-DY2, the pectinase produced by DY2. GC-MS based metabolomics was used to explore metabolic mechanisms mediated by glucose, showing the frustrated TCA cycle is necessary to elevate the activity and production of P-DY2. Decreased activity of α-ketoglutaric dehydrogenase and succinate dehydrogenase of DY2 in glucose-treated samples supports the conclusion that P-DY2 production is the TCA cycle-independent. These results reveal a metabolic mechanism of high-activity pectinase mediated by exogenous glucose. These findings highlight the way to understand metabolic mechanisms and promote pectinase yield through metabolomics approach and metabolic modulation, respectively.
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影响因子：
16.6
作者：
Earl DC;Ferrell PB Jr;Leelatian N;Froese JT;Reisman BJ;Irish JM;Bachmann BO
通讯作者：
Bachmann BO
影响因子：
4.7
作者：
Xian-liang Zhao;Yi Han;Shi-Tong Ren;Yan-mei Ma;Hui Li;Xuan-xian Peng
通讯作者：
Xian-liang Zhao;Yi Han;Shi-Tong Ren;Yan-mei Ma;Hui Li;Xuan-xian Peng
DOI：
10.1016/j.jrras.2015.11.003
发表时间：
2016-04-01
影响因子：
1.7
作者：
Ahmed, Ishtiaq;Zia, Muhammad Anjum;Nowrouzi, Azin
通讯作者：
Nowrouzi, Azin
增强的 TCA 循环可提高斑马鱼对溶藻弧菌感染的存活率
影响因子：
5.2
作者：
Yang MJ;Cheng ZX;Jiang M;Zeng ZH;Peng B;Peng XX;Li H
通讯作者：
Li H
DOI：
10.1007/s11157-017-9437-y
发表时间：
2017-07
期刊：
Reviews in Environmental Science and Bio/Technology
影响因子：
--
作者：
S. Rebello;M. Anju;E. Aneesh;R. Sindhu;P. Binod;Ashok Pandey
通讯作者：
S. Rebello;M. Anju;E. Aneesh;R. Sindhu;P. Binod;Ashok Pandey