Prostaglandin F2α-Induced Changes in the Sex Organs of the Male Laboratory Mouse
Prostaglandin F2α-Induced Changes in the Sex Organs of the Male Laboratory Mouse
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前列腺素 F2α 引起的雄性实验小鼠性器官的变化
DOI：
10.1055/s-0029-1210611
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发表时间：
2009
影响因子：
1.8
通讯作者：
C. Dominic
中科院分区：
文献类型：
--
作者：
Shio Kumar Singh;C. Dominic
：服用前列腺素 F2 α (PGF2 α)（3 毫克/公斤体重/天，持续 21 天）会显着抑制精子发生，并显着降低睾丸、附睾和副性腺的重量。生精小管没有精子，只含有支持细胞、精原细胞、精母细胞，偶尔还有精子细胞；在肾小管管腔内观察到几个多核巨细胞。间质细胞萎缩。然而，睾丸中 RNA、DNA 和蛋白质的水平并未受到药物治疗的影响。在接受药物治疗的小鼠中，附睾上皮呈现退化的外观；管腔通常没有精子，主要含有脱落的未成熟生殖细胞和精子碎片；附睾尾部精子即使存在，也是不动且破碎的。 PGF2α治疗还导致附睾头和尾部唾液酸水平以及精囊中果糖水平显着降低。结果表明，PGF2α引起的小鼠生殖器官的退化变化是由于干扰雄激素的分泌所致。施用PGF2α引起的生殖器官的改变是可逆的，停药后56天，器官恢复到正常状态。
: Administration (3 mg/kg body weight/day, for 21 days) of prostaglandin F2 alpha (PGF2 alpha) caused marked suppression of spermatogenesis and significant reduction in the weights of the testis, epididymis and accessory sex glands. The seminiferous tubules were devoid of spermatozoa and contained only Sertoli cells, spermatogonia, spermatocytes and occasionally spermatids; several multinucleated giant cells were observed in the lumen of the tubules. The Leydig cells were atrophied. The levels of RNA, DNA and protein in the testis were, however, unaffected by drug therapy. In drug-treated mice the epididymal epithelium presented a degenerate appearance; the lumen was generally devoid of spermatozoa and contained mainly exfoliated immature germ cells and sperm debris; cauda epididymidal spermatozoa, when present, were immotile and fragmented. PGF2 alpha treatment also caused significant decrease in the levels of sialic acid in the caput and cauda epididymides and of fructose in the seminal vesicle. The results suggest that the regressive changes induced by PGF2 alpha in the reproductive organs of the mouse are due to the interference with the secretion of androgen. The alterations induced in the reproductive organs by administration of PGF2 alpha were reversible and 56 days after drug withdrawal the organs returned to their normal state.