Expression of integrin cell adhesion receptors during human airway epithelial repair in vivo.
Expression of integrin cell adhesion receptors during human airway epithelial repair in vivo.
复制标题
体内人气道上皮修复过程中整合素细胞粘附受体的表达。
DOI：
10.1152/ajplung.1997.273.1.l256
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Albelda,SM
中科院分区：
文献类型：
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作者：
Pilewski,JM;Latoche,JD;Arcasoy,SM;Albelda,SM
气道上皮在炎症和暴露于各种吸入性和感染性因子期间易受损伤。关于整合素在人气道上皮损伤后再生和分化过程中的表达知之甚少。因此，我们在人支气管上皮的体内异种移植模型中表征了机械损伤后整合素亚基的表达。在表面上皮伤口边缘的迁移细胞上，α v-、β 5-、β 6-和α 5-整合素亚基的表达增加。在修复的后期，α v-，β 5-和β 6-亚基的表达持续增加，但β 8-亚基的表达仅限于基底细胞。此外，α 2-和α 6-胶原/层粘连蛋白结合整合素重新分布到基底上上皮层。修复上皮上没有β 3-或α 4-整合素亚基的表达。在囊性纤维化患者的未分化气道区域观察到α v-、β 5-和β 6-整合素受体亚单位的类似上调。发现损伤的上皮细胞对重组腺病毒的基因转移明显更敏感，这表明整合素表达的增加对腺病毒感染的获得和肺定向基因治疗具有意义。
Airway epithelium is subject to injury during inflammation and exposure to a variety of inhaled and infectious agents. Little is known about the expression of integrins during human airway epithelial regeneration and differentiation after injury. We therefore characterized integrin subunit expression after mechanical injury in an in vivo xenograft model of human bronchial epithelium. On the migrating cells at the edges of surface epithelial wounds, there was increased expression of the alpha v-, beta 5-, beta 6-, and alpha 5-integrin subunits. During the later phase of repair, the increased expression of alpha v-, beta 5-, and beta 6-subunits persisted, but the expression of the beta 8-subunits was restricted to basal cells. In addition, there was a redistribution of the alpha 2- and alpha 6-collagen/laminin-binding integrins to suprabasal epithelial layers. There was no expression of the beta 3- or alpha 4-integrin subunit on reparative epithelium. A similar upregulation of alpha v-, beta 5-, and beta 6-integrin receptor subunits was observed in areas of undifferentiated airway from cystic fibrosis patients. Injured epithelium was found to be significantly more susceptible to gene transfer with a recombinant adenovirus, suggesting that the increased integrin expression has implications for the acquisition of adenovirus infections and for lung-directed gene therapy.