Gene expression of group II phospholipase A2 in intestine in ulcerative colitis
Gene expression of group II phospholipase A2 in intestine in ulcerative colitis
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DOI：
10.1136/gut.40.1.95
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发表时间：
1997-01-01
期刊：
影响因子：
24.5
通讯作者：
Nevalainen, TJ
中科院分区：
文献类型：
--
作者：
Haapamaki, MM;Gronroos, JM;Nevalainen, TJ
背景-磷脂酶A2（PLA 2）在炎症性肠病的发病机制中具有重要作用。目的-本研究旨在鉴定溃疡性结肠炎患者肠和肠系膜组织样本中可能在mRNA和酶蛋白水平表达II型磷脂酶A2（PLA 2-II）的细胞。患者和组织样本-组织样本从肠、肠系膜、骨骼肌和皮下脂肪的六名患者接受了全直肠结肠切除术的严重溃疡性结肠炎。在常规诊断性结肠镜检查中，从另一组6例溃疡性结肠炎患者的结肠中获得粘液活检标本。方法：组织样本通过光学显微镜，免疫组织化学方法检测PLA 2-II酶蛋白，用原位杂交和北方杂交检测PLA 2-Ⅱ mRNA。在6例患者的化生潘氏细胞中检测到II蛋白，在6例结肠癌患者中的4例结肠粘膜柱状上皮细胞中检测到II蛋白。活动性溃疡性结肠炎轻度溃疡性结肠炎患者的阳性结果较少。仅2/6例对照组患者的PLA 2-II mRNA呈弱阳性信号，其中1例患者的结肠粘膜柱状上皮细胞中PLA 2-II呈弱阳性免疫反应。对照组患者无化生Paneth细胞。结论化生Paneth细胞和结肠上皮细胞合成PLA 2-II在溃疡性结肠炎。PLA 2-II合成的活性似乎与患病肠道中的炎症程度有关。
Background-It has been suggested that phospholipase A2 (PLA2) has an essential role in the pathogenesis of inflammatory bowel diseases.Aims-This study aimed at identifying cells in intestinal and mesenteric tissue samples that might express group II phospholipase A2 (PLA2-II) at the mRNA and enzyme protein levels in patients with ulcerative colitis.Patients and tissue samples-Tissue samples were obtained from the intestine, mesentery, skeletal muscle, and subcutaneous fat of six patients who underwent panproctocolectomy for severe ulcerative colitis. Mucosal biopsy specimens were obtained from the colon of another group of six patients with ulcerative colitis during routine diagnostic colonoscopies. Tissues from six patients without intestinal inflammatory diseases served as controls.Methods-Tissue samples were studied by light microscopy, immunohistochemistry for PLA2-II enzyme protein, and in situ hybridisation and northern hybridisation for PLA2-II mRNA.Results-PLA2-II mRNA and PLA2-II protein were detected in metaplastic Paneth cells in six patients and in the columnar epithelial cells of colonic mucosa in four out of six patients with active ulcerative colitis. Positive findings were less numerous in patients with mild ulcerative colitis. Only two out of six control patients had a weak positive signal for PLA2-II mRNA and one of these two patients had a weak positive immunoreaction for PLA2-II in columnar epithelial cells in the colonic mucosa. None of the control patients had metaplastic Paneth cells.Conclusions-Metaplastic Paneth cells and colonic epithelial cells synthesise PLA2-II in ulcerative colitis. The activity of the PLA2-II synthesis seems to be related to the degree of inflammation in the diseased bowel.