THE MOLECULAR-BASIS OF P-M HYBRID DYSGENESIS - THE NATURE OF INDUCED MUTATIONS
THE MOLECULAR-BASIS OF P-M HYBRID DYSGENESIS - THE NATURE OF INDUCED MUTATIONS
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DOI：
10.1016/0092-8674(82)90462-7
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发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
BINGHAM, PM
中科院分区：
文献类型：
--
作者：
RUBIN, GM;KIDWELL, MG;BINGHAM, PM
研究了黑腹果蝇（Drosophilamelanogaster）P株和M株之间的基因缺陷杂种中突变的分子本质。在白色基因座的7个独立的突变进行了检查，这些落入2类的基础上，他们的遗传和结构特性。构成第1类的5个突变分别由0.5、0.5、0.6、1.2和1.4 kb的DNA插入引起。详细检查了其中4个突变中的DNA插入。虽然限制性内切酶位点的大小和模式不同，但它们在序列上是同源的。该序列家族的成员被称为P元件。由P元件引起的突变在P细胞型中似乎是稳定的，但在M细胞型中具有大于10-3的回复率。表型回复到野生型伴随着切除的P元素。第2类的2个突变是由插入5.0 kb copia元件引起的，在P和M细胞型中似乎都是稳定的。
The molecular nature of mutations arising in dysgenic hybrids between P and M Drosophila melanogaster strains was investigated. Seven independent mutations at the white locus were examined, and these fell into 2 classes on the basis of their genetic and structural properties. The 5 mutations comprising the 1st class were caused by DNA insertions of 0.5, 0.5, 0.6, 1.2 and 1.4 kb [kilobase], respectively. The DNA insertions in 4 of these mutations were examined in detail. Although heterogeneous in size and pattern of restriction enzyme sites, they were homologous in sequence. Members of this sequence family are referred to as P elements. Mutations caused by P elements appeared to be stable in the P cytotype, but had reversion rates greater than 10-3 in the M cytotype. Phenotypic reversion to wild-type was accompanied by excision of the P element. The 2 mutations comprising the 2nd class were caused by insertion of the 5.0 kb copia element and appeared to be stable in both P and M cytotypes.